通过荧光抗体染色法测定产黑色素拟杆菌血清群
Determination of Bacteroides melaninogenicus serogroups by fluorescent antibody staining.
作者信息
Lambe D W
出版信息
Appl Microbiol. 1974 Oct;28(4):561-7. doi: 10.1128/am.28.4.561-567.1974.
Abstract
Fluorescein isothiocyanate-labeled antibody reagents (conjugates) were prepared to one strain of each of the three subspecies of Bacteroides melaninogenicus: B. melaninogenicus subsp. melaninogenicus, B. melaninogenicus subsp. asaccharolyticus, and B. melaninogenicus subsp. intermedius. These three conjugates were specific; thus, they provided a new serological classification of B. melaninogenicus. The three serogroups were designated A, B, and C. Most test strains (98%) isolated from human clinical specimens were assigned to a specific serogroup by immunofluorescence, and the serogroup of these test strains corroborated the biochemical characterization of the three subspecies of B. melaninogenicus. The conjugates failed to cross-react with other anaerobes or aerobes tested. This fluorescent antibody technique provided a more rapid classification of the three subspecies of B. melaninogenicus than did conventional biochemical methods.
摘要
制备了异硫氰酸荧光素标记的抗体试剂（结合物），用于产黑素拟杆菌三个亚种中的每一个亚种的一个菌株：产黑素拟杆菌产黑素亚种、产黑素拟杆菌解糖亚种和产黑素拟杆菌中间亚种。这三种结合物具有特异性；因此，它们为产黑素拟杆菌提供了一种新的血清学分类。这三个血清群分别命名为A、B和C。通过免疫荧光法，从人类临床标本中分离出的大多数测试菌株（98%）被归入特定的血清群，这些测试菌株的血清群证实了产黑素拟杆菌三个亚种的生化特征。这些结合物与所测试的其他厌氧菌或需氧菌没有交叉反应。与传统生化方法相比，这种荧光抗体技术为产黑素拟杆菌的三个亚种提供了更快速的分类。
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