Nadi N S, Hirsch J D, Margolis F L
J Neurochem. 1980 Jan;34(1):138-46. doi: 10.1111/j.1471-4159.1980.tb04632.x.
Coronal sections of frozen dog olfactory bulb have been dissected into four anatomically distinct layers. The laminar distribution of ten amino acids, the dipeptide carnosine, and nine [3H]ligand binding sites in these layers was determined. GABA and tyrosine levels were highest in the mitral cell-granule cell layer, and glutamate levels were slightly elevated in the glomerular layer. The distributions of all other amino acids did not show significant differences across the layers. Carnosine was predominantly localized in the fiber and glomerular layers. With the exception of quinuclidinyl benzilate, the [3H]ligand binding sites showed more discrete distributions. Muscimol, diazepam, kainic acid, and spiroperidol binding were predominantly localized in the mitral cell-granule cell layer, where clonidine binding was at a minimum. Dihydromorphine binding was high in both the fiber and the mitral cell-granule cell layers. Carnosine binding was maximal in the glomerular layer. The implications of these observations with regard to biochemical and neurophysiological data are discussed.
已将冷冻犬嗅球的冠状切片解剖为四个解剖学上不同的层。测定了这几层中十种氨基酸、二肽肌肽和九个[3H]配体结合位点的层状分布。GABA和酪氨酸水平在二尖瓣细胞-颗粒细胞层中最高，而谷氨酸水平在肾小球层中略有升高。所有其他氨基酸的分布在各层之间没有显示出显著差异。肌肽主要定位于纤维层和肾小球层。除了奎宁环基苯甲酸酯外，[3H]配体结合位点显示出更离散的分布。蝇蕈醇、地西泮、 kainic酸和螺哌啶醇的结合主要定位于二尖瓣细胞-颗粒细胞层，而可乐定结合最少。二氢吗啡结合在纤维层和二尖瓣细胞-颗粒细胞层中都很高。肌肽结合在肾小球层中最大。讨论了这些观察结果对生化和神经生理学数据的影响。
