大鼠下丘脑生长抑素-14的分离与鉴定

Isolation and characterization of rat hypothalamic somatostatin-14.

作者信息

Bohlen P, Benoit R, Ling N, Guillemin R, Brazeau P

出版信息

Endocrinology. 1981 May;108(5):2008-10. doi: 10.1210/endo-108-5-2008.

DOI:10.1210/endo-108-5-2008

Abstract

A peptide with somatostatin-like immuno- and bioactivity has been isolated from 1165 rat hypothalami by using antisomatostatin affinity chromatography, gel filtration and reverse-phase high-performance liquid chromatography. The isolated peptide is indistinguishable from synthetic somatostatin-14 with respect to chromatographic properties and amino acid composition. We therefore propose that rat hypothalamic somatostatin-14 is identical in structure to somatostatin-14 found in other species.

摘要

利用抗生长抑素亲和层析、凝胶过滤和反相高效液相色谱法，从1165个大鼠下丘脑分离出一种具有生长抑素样免疫活性和生物活性的肽。就色谱性质和氨基酸组成而言，分离出的肽与合成的生长抑素-14无法区分。因此，我们认为大鼠下丘脑生长抑素-14在结构上与其他物种中发现的生长抑素-14相同。