Hasegawa Y, Miyamoto K, Nomura M, Igarashi M, Kangawa K, Matsuo H
Endocrinology. 1984 Jul;115(1):433-5. doi: 10.1210/endo-115-1-433.
Four peptides having SRIF-like bioactivity when assayed by a rat anterior pituitary cell culture system have been isolated from chicken hypothalamic extracts. These four peptides have been purified from 2,000-5,000 chicken hypothalami in a yield of 2.2 nmol to 16 nmol. The isolated SRIFs have been characterized by amino acid analysis. One consists of 28 amino acids which are identical with mammalian SRIF-28 except that one Met residue is replaced by a Leu residue. Three other SRIF-like substances with low mol wt have also been isolated in a pure state. One is identical with mammalian SRIF-14, as judged by chromatographic comparison on HPLC and its amino acid composition as well as the specific bioactivity on rat pituitary cells. The amino acid composition and specific bioactivity of another one is identical with that of mammalian SRIF-14, but it is different from mammalian SRIF-14 in its elution position on HPLC. The third SRIF-like substance consists of 16 amino acids and is less potent than mammalian SRIF-14. It is concluded that several SRIF-like peptides exist and that SRIF-28, with one amino acid substitution from Met to Leu, and SRIF-14, identical with the mammalian one, are the major components in chicken hypothalamus.
通过大鼠垂体前叶细胞培养系统检测，已从鸡下丘脑提取物中分离出四种具有生长抑素（SRIF）样生物活性的肽。这四种肽是从2000至5000个鸡下丘脑中纯化得到的，产量为2.2纳摩尔至16纳摩尔。分离出的生长抑素已通过氨基酸分析进行了表征。其中一种由28个氨基酸组成，除一个甲硫氨酸残基被亮氨酸残基取代外，与哺乳动物的生长抑素-28相同。另外三种低分子量的生长抑素样物质也已被纯态分离出来。通过高效液相色谱（HPLC）的色谱比较、其氨基酸组成以及对大鼠垂体细胞的特定生物活性判断，其中一种与哺乳动物的生长抑素-14相同。另一种的氨基酸组成和特定生物活性与哺乳动物的生长抑素-14相同，但在HPLC上的洗脱位置与哺乳动物的生长抑素-14不同。第三种生长抑素样物质由16个氨基酸组成，活性比哺乳动物的生长抑素-14弱。得出的结论是，存在几种生长抑素样肽，并且甲硫氨酸被亮氨酸取代一个氨基酸的生长抑素-28以及与哺乳动物的生长抑素-14相同的生长抑素-14是鸡下丘脑中的主要成分。
