实验性肾盂肾炎中对大肠杆菌菌毛的局部免疫反应
Local immune response to Escherichia coli pili in experimental pyelonephritis.
作者信息
Smith J W, Wagner S, Swenson R M
出版信息
Infect Immun. 1981 Jan;31(1):17-20. doi: 10.1128/iai.31.1.17-20.1981.
The local immune response to pili of Escherichia coli O6:K13:H1 was determined in experimental hematogenous pyelonephritis in rabbits. Pili purified from sheared cells by ammonium sulfate precipitation were found to be pure by electron microscopy and negative for lipopolysaccharide by limulus lysate assay. Antipilus antibody was detected in serum and newly synthesized protein from infected animals with enzyme-linked immunosorbent assay. Serum and local (intrarenal) antibodies were of the immunoglobulin G class, were detectable by day 20 of infection, and persisted though 250 days of infection. These data suggest that pili are present on the organism at the site of infection, since they induce the local synthesis of antipilus antibody in experimental pyelonephritis.
在兔实验性血源性肾盂肾炎中，测定了对大肠杆菌O6:K13:H1菌毛的局部免疫反应。通过硫酸铵沉淀从剪切细胞中纯化的菌毛，经电子显微镜检查发现是纯的，鲎试剂检测显示脂多糖呈阴性。用酶联免疫吸附测定法在感染动物的血清和新合成蛋白中检测到抗菌毛抗体。血清和局部（肾内）抗体均为免疫球蛋白G类，在感染第20天可检测到，并在感染250天期间持续存在。这些数据表明，菌毛存在于感染部位的细菌上，因为它们在实验性肾盂肾炎中诱导了抗菌毛抗体的局部合成。
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