Perry A, Ofek I, Silverblatt F J
Infect Immun. 1983 Mar;39(3):1334-45. doi: 10.1128/iai.39.3.1334-1345.1983.
In the present study, we assayed protein iodination in human granulocytes after interaction of the cells with mannose-specific (MS) type 1 fimbriated (MS+) and nonfimbriated (MS-) phenotypes of Escherichia coli pretreated with various amounts of anti-E. coli and antifimbrial antibodies. The MS+ phenotype stimulated protein iodination in granulocytes and possessed potent MS activity as measured by yeast aggregometry. In contrast, the MS- phenotype lacked all these activities. MS+ pretreated with moderate concentrations of antibodies, however, showed up to a 15-fold increase in granulocyte stimulation as compared to granulocyte stimulation induced by the non-antibody-treated MS+ phenotype or by the antibody-treated MS- phenotype. This marked antibody-mediated increase in stimulation of granulocytes was (i) dependent on the antibody concentrations, (ii) markedly reduced by methyl-alpha-D-mannoside, (iii) caused by immunoglobulin G as well as by F(ab')2, (iv) caused only by antifimbrial antibodies, (v) associated with cross-linked fimbriae seen as "bundles" under an electron microscope, and (vi) mimicked by treating MS+ bacteria with a certain range of glutaraldehyde. The data taken together support the hypothesis that, although cross-linking of fimbriae reduced the density of functional MS fimbriae over the surface of antibody-treated bacteria and consequently reduced the ability of these organisms to agglutinate yeast cells, the resulting bundles of MS fimbriae were far more effective at stimulating granulocytes because, bound together, they were better equipped to aggregate the mannose-containing receptors on the granulocyte surface.
在本研究中，我们检测了人粒细胞中的蛋白质碘化作用，这些粒细胞与用不同量的抗大肠杆菌和抗菌毛抗体预处理过的大肠杆菌的甘露糖特异性（MS）1型菌毛化（MS+）和非菌毛化（MS-）表型相互作用之后。MS+表型刺激粒细胞中的蛋白质碘化作用，并且如通过酵母凝集试验所测定的那样，具有很强的MS活性。相比之下，MS-表型缺乏所有这些活性。然而，用中等浓度抗体预处理的MS+与未用抗体处理的MS+表型或用抗体处理的MS-表型诱导的粒细胞刺激相比，显示出粒细胞刺激增加了高达15倍。这种由抗体介导的显著的粒细胞刺激增加（i）取决于抗体浓度，（ii）被α-D-甲基甘露糖苷显著降低，（iii）由免疫球蛋白G以及F(ab')2引起，（iv）仅由抗菌毛抗体引起，（v）与在电子显微镜下可见为“束”状的交联菌毛有关，并且（vi）通过用一定范围的戊二醛处理MS+细菌来模拟。综合这些数据支持这样的假说，即尽管菌毛交联降低了抗体处理过的细菌表面功能性MS菌毛的密度，从而降低了这些生物体凝集酵母细胞的能力，但由此产生的MS菌毛束在刺激粒细胞方面要有效得多，因为它们结合在一起时，能更好地聚集粒细胞表面含甘露糖的受体。
