Cassiman J J, Verlinden J, Van der Schueren B, Van Leuven F, Saison M, Van den Berghe H
Virchows Arch B Cell Pathol Incl Mol Pathol. 1981;37(2):179-89. doi: 10.1007/BF02892566.
The cellular distribution of a highly antigenic fibroblast glycoprotein, identified previously as aminopeptidase M, was studied in vitro by immunofluorescence and immuno-electron microscopy. The antigen was found to be localized at the surface of live and fixed fibroblasts in suspension and in cell layer; clustering or patching on live cells could be observed. Immunofluorescence after permeabilization of the cells with acetone showed distribution of the antigen in cytoplasmic granules. The tissue distribution of this antigen was examined by immunofluorescence on frozen sections of various human organs. In addition to fibroblasts, renal tubules, liver, pancreas and gut were found to react selectively with the antibody preparation. Moreover, a specific localization of the reaction product was observed. In the kidney the epithelium and brush border of the proximal convoluted tubules were stained. In the liver, the bile canaliculi reacted; in the pancreas the acinar cells and in the gut the brush border of the mucosal cells of the villi and crypts were stained. The same cells did not stain with an antiserum prepared in a similar fashion against another fibroblast component, or with preimmune IgG. In most sections of the selectively stained tissues, a predominant localization at the apical pole of the cells was observed. The localization of the antigen in different tissues corresponds with the distribution of aminopeptidase M, thereby confirming its identity and the antigenic cross-reaction between the fibroblast and tissue enzyme.
一种先前被鉴定为氨肽酶M的高抗原性成纤维细胞糖蛋白的细胞分布，通过免疫荧光和免疫电子显微镜在体外进行了研究。发现该抗原定位于悬浮液和细胞层中活的和固定的成纤维细胞表面；可观察到活细胞上出现聚集或斑块状。用丙酮使细胞通透后进行免疫荧光显示抗原分布于细胞质颗粒中。通过对各种人体器官冷冻切片进行免疫荧光检查了该抗原的组织分布。除成纤维细胞外，还发现肾小管、肝脏、胰腺和肠道与抗体制剂发生选择性反应。此外，观察到反应产物的特异性定位。在肾脏中，近端曲管的上皮和刷状缘被染色。在肝脏中，胆小管发生反应；在胰腺中，腺泡细胞以及在肠道中，绒毛和隐窝的粘膜细胞的刷状缘被染色。同样的细胞用针对另一种成纤维细胞成分以类似方式制备的抗血清或用免疫前IgG染色均无反应。在选择性染色组织的大多数切片中，观察到抗原主要定位于细胞的顶端极。抗原在不同组织中的定位与氨肽酶M的分布一致，从而证实了其同一性以及成纤维细胞和组织酶之间的抗原交叉反应。
