Wachsmuth E D
Acta Histochem Suppl. 1976;16:221-31.
The method and instrumentation for the analysis of enzyme activity and of fluorescent antibody of antigen binding is described. It is based on the microscopic photometry of stain intensities per area of functional units. Thus it was shown that the concentration of aminopeptidase (EC 3.4.2.1) per area in procimal tubule of kidney from pig and mouse is constant. In contrast to the pig the tubules from the outer medulla of the mouse contained twice as much enzyme as those from the cortex. A non constant concentration of aminopeptidase per area was found in crypts of duodenum and in stimulated macrophages.
本文描述了分析酶活性和抗原结合荧光抗体的方法及仪器。它基于对功能单位每单位面积染色强度的显微光度测定。结果表明，猪和小鼠肾脏近端小管每单位面积的氨肽酶（EC 3.4.2.1）浓度是恒定的。与猪不同，小鼠外髓质的小管所含酶量是皮质小管的两倍。在十二指肠隐窝和受刺激的巨噬细胞中发现氨肽酶每单位面积的浓度不恒定。
