Jan L Y, Jan Y N
Fed Proc. 1981 Sep;40(11):2560-4.
In sympathetic ganglia of the bullfrog a peptide that resembles luteinizing hormone releasing hormone (LHRH) probably functions as the transmitter for the late slow excitatory postsynaptic potential (EPSP), a signal that lasts several minutes: 1) Radioimmunoassays indicated that the lumbar chain of sympathetic ganglia contains 100-800 pg of an LHRH-like peptide, which disappeared 5 days after preganglionic axons were cut, while by this time the LHRH-like immunoreactivity tripled in the spinal nerves proximal to the cut region. 2) Immunohistochemical studies demonstrated the presence of LHRH-like immunoreactivity in numerous nerve terminals around sympathetic neurons. 3) The LHRH-like peptide can be released from ganglia with isotonic KCI or with nerve stimulation, a release that requires Ca2+. 4) LHRH acts directly on sympathetic neurons and produces a depolarizing response lasting for minutes. 5) The LHRH response and the late slow EPSP are associated with similar membrane conductance changes. Further, they alter the excitability of the neuron and interact with the cholinergic EPSPs in a parallel manner. 6) Both responses are blocked by certain analogs of LHRH, which antagonize the effects of LHRH in the rat.
在牛蛙的交感神经节中，一种类似于促黄体生成素释放激素（LHRH）的肽可能作为晚期缓慢兴奋性突触后电位（EPSP）的递质，该信号持续数分钟：1）放射免疫分析表明，交感神经节的腰链含有100 - 800皮克的LHRH样肽，在节前轴突切断5天后消失，而此时在切断区域近端的脊神经中，LHRH样免疫反应性增加了两倍。2）免疫组织化学研究表明，在交感神经元周围的许多神经末梢中存在LHRH样免疫反应性。3）LHRH样肽可通过等渗氯化钾或神经刺激从神经节释放，这种释放需要钙离子。4）LHRH直接作用于交感神经元并产生持续数分钟的去极化反应。5）LHRH反应和晚期缓慢EPSP与类似的膜电导变化相关。此外，它们改变神经元的兴奋性，并以平行方式与胆碱能EPSP相互作用。6）两种反应均被某些LHRH类似物阻断，这些类似物可拮抗LHRH在大鼠中的作用。
