新皮质组织亚细胞成分中的环磷酸腺苷结合能力与组蛋白激酶激活。对三种神经体液因子的不同反应。
Cyclic AMP-binding capacities and histone kinase activation in subcellular components of neocortical tissue. Differential responses to three neurohumoural agents.
作者信息
Patel J, Newman M, McIlwain H
出版信息
Biochem J. 1981 Feb 15;194(2):621-6. doi: 10.1042/bj1940621.
Abstract
- Noradrenaline and histamine, when added to superfused guinea-pig cerebral-cortical tissues, increased both cyclic AMP-dependent and -independent histone kinase activities of some, but not of all, subsequently isolated subcellular fractions, and decreased their cyclic [(3)H]AMP-binding capacity, which was concluded to be due to an increase in endogenously bound cyclic AMP. 2. Adenosine and 2-chloroadenosine also diminished the cyclic [(3)H]AMP-binding capacities, but did not affect the histone kinase activities. 3. DEAE-cellulose chromatography and stability to KCl additions showed that the greater part of the histone kinase of the present preparations corresponded to the type II enzyme [of Corbin, Keely & Park (1975) J. Biol. Chem.250, 218-225], with a lesser amount of type I activity. Different sites of cyclic AMP accumulation in relation to these or other kinases are considered in interpreting the differential tissue responses to the neurohumoural agents examined.
摘要
- 去甲肾上腺素和组胺添加到灌流的豚鼠大脑皮质组织中时，会增加部分（而非全部）随后分离出的亚细胞组分中依赖环磷酸腺苷（cAMP）和不依赖cAMP的组蛋白激酶活性，并降低其环[³H]AMP结合能力，得出这是由于内源性结合的环磷酸腺苷增加所致。2. 腺苷和2 - 氯腺苷也会降低环[³H]AMP结合能力，但不影响组蛋白激酶活性。3. 二乙氨基乙基纤维素色谱分析以及对添加氯化钾的稳定性表明，本制剂中大部分组蛋白激酶对应于[科尔宾、基利和帕克（1975年）《生物化学杂志》250, 218 - 225]的II型酶，I型活性较少。在解释对所研究的神经体液因子的不同组织反应时，考虑了与这些或其他激酶相关的环磷酸腺苷积累的不同位点。
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本文引用的文献
1
The binding of [3H]adenosine to synaptosomal and other preparations from the mammalian brain.
Biochem J. 1981 Feb 15;194(2):611-20. doi: 10.1042/bj1940611.
2
Adenosine-binding to cerebral preparations in interpretation of adenosine activation of adenosine 3':5'-cyclic monophosphate formation [proceedings].
Biochem Soc Trans. 1980 Feb;8(1):141-2. doi: 10.1042/bst0080141.
3
Stimulation of piriform- and neo-cortical tissues in an in vitro flow-system: metabolic properties and release of putative neurotransmitters.
J Neurochem. 1970 Apr;17(4):521-30. doi: 10.1111/j.1471-4159.1970.tb00530.x.
4
Depolarization-evoked accumulation of cyclic AMP in brain slices: inhibition by exogenous adenosine deaminase.
Biochim Biophys Acta. 1973 Nov 2;329(1):147-51. doi: 10.1016/0304-4165(73)90017-2.
5
Intracellular titration of cyclic AMP bound to receptor proteins and correlation with cyclic-AMP levels in the surviving rat diaphragm.
Eur J Biochem. 1973 Dec 3;40(1):177-85. doi: 10.1111/j.1432-1033.1973.tb03183.x.
6
The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices.
Mol Pharmacol. 1970 Jan;6(1):13-23.
7
Protein kinase activity in membrane preparations from ox brain. Stimulation of intrinsic activity by adenosine 3':5'-cyclic monophosphate.
Biochem J. 1973 Mar;132(3):483-92. doi: 10.1042/bj1320483.
8
Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents.
Biochem J. 1973 Mar;132(3):475-82. doi: 10.1042/bj1320475.
9
Regulation of adenosine 3',5'-monophosphate-dependent protein kinase. I. Preliminary characterization of the adipose tissue enzyme in crude extracts.
J Biol Chem. 1973 Mar 10;248(5):1813-21.
10
Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.
J Biol Chem. 1971 Apr 10;246(7):1977-85.
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