Schnitzer-Polokoff R, von Gunten C, Logel J, Torget R, Sinensky M
J Biol Chem. 1982 Jan 10;257(1):472-6.
A somatic cell mutant of the Chinese hamster ovary (CHO)-K1 cell auxotrophic for mevalonic acid has been isolated by means of the bromodeoxyuridine-visible light technique. This mutant can incorporate labeled mevalonate but not labeled acetate into cholesterol and, thus, is apparently defective in mevalonate biosynthesis. The mutant is recessive with respect to the parental cell phenotype. Assessment of the in vitro activities of the enzymes responsible for mevalonate biosynthesis under varying growth conditions indicates that the mutant, Mev-1, is defective in 3-hydroxy-3-methylglutaryl coenzyme A synthase.
利用溴脱氧尿苷-可见光技术分离出了中国仓鼠卵巢（CHO）-K1细胞的一种体细胞突变体，该突变体对甲羟戊酸营养缺陷。这种突变体能够将标记的甲羟戊酸掺入胆固醇中，但不能将标记的乙酸盐掺入胆固醇中，因此，其甲羟戊酸生物合成显然存在缺陷。该突变体相对于亲本表型是隐性的。在不同生长条件下对负责甲羟戊酸生物合成的酶的体外活性进行评估表明，突变体Mev-1在3-羟基-3-甲基戊二酰辅酶A合酶方面存在缺陷。
