Herbschleb-Voogt E, Grzeschik K H, Pearson P L, Meera Khan P
Hum Genet. 1981;59(4):317-23. doi: 10.1007/BF00295464.
The experiments reported in this paper indicate that the expression of human adenosine deaminase complexing protein (ADCP) in the human-rodent somatic cell hybrids is influenced by the state of confluency of the cells and the background rodent genome. Thus, the complement of the L-cell derived A9 or B82 mouse parent apparently prevents the expression of human ADCP in the interspecific somatic cell hybrids. In the a3, E36, or RAG hybrids the human ADCP expression was not prevented by the rodent genome and was found to be proportional to the degree of confluency of the cell in the culture as in the case of primary human fibroblasts. An analysis of human chromosomes, chromosome specific enzyme markers, and ADCP in a panel of rodent-human somatic cell hybrids optimally maintained and harvested at full confluency has shown that the expression of human ADCP in the mouse (RAG)-human as well as in the hamster (E36 or a3)-human hybrids is determined by a gene(s) in human chromosome 2 and that neither chromosome 6 nor any other of the chromosomes of man carry any gene(s) involved in the formation of human ADCP at least in the Chinese hamster-human hybrids. A series of rodent-human hybrid clones exhibiting a mitotic separation of IDH1 and MDH1 indicated that ADCP is most probably situated between corresponding loci in human chromosome 2.
本文报道的实验表明，人腺苷脱氨酶复合蛋白（ADCP）在人 - 啮齿动物体细胞杂种中的表达受细胞汇合状态和背景啮齿动物基因组的影响。因此，源自L细胞的A9或B82小鼠亲本的互补物显然会阻止人ADCP在种间体细胞杂种中的表达。在a3、E36或RAG杂种中，人ADCP的表达不受啮齿动物基因组的阻止，并且发现其与培养物中细胞的汇合程度成比例，就像原代人成纤维细胞的情况一样。对一组在完全汇合时最佳维持和收获的啮齿动物 - 人体细胞杂种中的人类染色体、染色体特异性酶标记和ADCP进行分析表明，人ADCP在小鼠（RAG） - 人以及仓鼠（E36或a3） - 人杂种中的表达由人类染色体2中的一个基因决定，并且至少在中国仓鼠 - 人杂种中，染色体6或人类的任何其他染色体都不携带参与人ADCP形成的任何基因。一系列表现出IDH1和MDH1有丝分裂分离的啮齿动物 - 人杂交克隆表明，ADCP很可能位于人类染色体2中的相应基因座之间。
