Willcox M B, Golde D W, Cline M J
J Histochem Cytochem. 1976 Sep;24(9):979-83. doi: 10.1177/24.9.61241.
Growth of human bone marrow in liquid suspension cultures has been used to study normal hematopoietic cell differentiation and abnormalities in blood diseases. A variety of cytochemical stains were applied to human marrow cells cultured in vitro for up to 14 days. AS-D- CHLOROACETATE ESTERASE AND ALPHA-NAPHYHYL BUTYrate esterase were most useful in distinguishing different cell lines in culture. Peroxidase activity disappeared with mononuclear phagocyte morphogenesis and diminished with culture in intermediate and mature granulocytes. Acid phosphatase activity and methyl greed pyronin staining intensity increased with macrophage maturation.
人类骨髓在液体悬浮培养中的生长已被用于研究正常造血细胞分化及血液疾病中的异常情况。将多种细胞化学染色应用于体外培养长达14天的人类骨髓细胞。AS-D-氯乙酸酯酶和α-萘丁酸酯酶在区分培养中的不同细胞系方面最有用。过氧化物酶活性随单核吞噬细胞形态发生而消失，并在中间和成熟粒细胞培养时降低。酸性磷酸酶活性和甲基绿派洛宁染色强度随巨噬细胞成熟而增加。
