Lee P W, Svedmyr A, Amyx H L, Gibbs C J, Gajdusek D C
Intervirology. 1982;18(1-2):38-44. doi: 10.1159/000149302.
A 'nonspecific' fluorescence probably caused by immunocomplexes appears in the lung tissue of immunocompetent hosts infected with Korean hemorrhagic fever (KHF) or epidemic nephropathy (EN) viruses. Indirect immunofluorescence tests with such KHF- or EN-infected tissue as antigen are unsatisfactory for the detection of low-titer antibody or the reading of titer endpoints. Treatment of acetone-fixed lung sections at pH 3.0 followed by washing with 0.01 M phosphate-buffered saline and distilled water apparently dissolves the immunocomplexes, resulting in an effective removal of the 'nonspecific' fluorescence while the viral antigens are not significantly impaired. Lung sections of KHF-infected Apodemus agrarius coreae and EN-infected Clethrionomys glareolus, acetone-fixed and treated at pH 3.0, were used for the antigenic differentiation of the EN and KHF viruses.
在感染朝鲜出血热（KHF）或流行性肾病（EN）病毒的免疫功能正常宿主的肺组织中，出现了一种可能由免疫复合物引起的“非特异性”荧光。以感染KHF或EN的组织为抗原进行间接免疫荧光试验，对于低滴度抗体的检测或滴度终点的判读并不理想。将丙酮固定的肺切片在pH 3.0条件下处理，然后用0.01M磷酸盐缓冲盐水和蒸馏水洗涤，显然可以溶解免疫复合物，从而有效去除“非特异性”荧光，而病毒抗原并未受到显著损害。用pH 3.0处理过的丙酮固定的感染KHF的黑线姬鼠和感染EN的棕背䶄的肺切片，用于EN和KHF病毒的抗原鉴别。
