Friman G, French G R, Hambraeus L, Beisel W R, Ilbäck N G
Scand J Infect Dis. 1981;13(2):89-93. doi: 10.3109/inf.1981.13.issue-2.02.
A newly developed spot slide immunofluorescence method utilizing an in vitro antigen source was used for the first time for the assay of antibodies reactant with the Korean haemorrhagic fever (KHF) agent in sera from patients diagnosed with Scandinavian epidemic (endemic) nephropathy (nephropathia epidemica, NE) and from age-matched control patients living in the same area as the NE patients but suffering from other diseases. KHF antibodies were demonstrated in all of 14 NE patients who were followed prospectively, 7 of whom exhibited seroconversion and in 6 of 8 NE patients studied retrospectively, but in only one of 42 controls. Antibodies in NE appeared within the first week of onset of symptoms and persisted for long periods of time as seen in KHF. The time from the onset of the illness until maximum antibody titre was recorded varied from 9 days to 1 month. On an average, the level of the antibody titres measured in NE was lower than that usually encountered in the Korean disease. The results indicate a close antigenic relationship between the KHF and NE agents and demonstrate that the reliability of our new spot slide method is similar to that of another previously reported and more laborious immunofluorescence method using lung from infected rodents as antigen source.
一种新开发的利用体外抗原源的斑点玻片免疫荧光法首次用于检测诊断为斯堪的纳维亚流行性（地方性）肾病（肾病综合征，NE）患者以及与NE患者居住在同一地区但患有其他疾病的年龄匹配对照患者血清中与韩国出血热（KHF）病原体发生反应的抗体。在14例接受前瞻性随访的NE患者中，所有患者均检测到KHF抗体，其中7例出现血清转化；在8例回顾性研究的NE患者中，有6例检测到KHF抗体，但在42例对照患者中，只有1例检测到KHF抗体。NE患者的抗体在症状出现的第一周内出现，并像在KHF中一样持续很长时间。从发病到记录到最高抗体滴度的时间从9天到1个月不等。平均而言，NE患者中测得的抗体滴度水平低于韩国疾病中通常遇到的水平。结果表明KHF和NE病原体之间存在密切的抗原关系，并证明我们新的斑点玻片法的可靠性与另一种先前报道的、使用感染啮齿动物的肺作为抗原源且更为繁琐的免疫荧光法相似。
