DNA介导的多重耐药性转移与质膜糖蛋白表达
DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.
作者信息
Debenham P G, Kartner N, Siminovitch L, Riordan J R, Ling V
出版信息
Mol Cell Biol. 1982 Aug;2(8):881-9. doi: 10.1128/mcb.2.8.881-889.1982.
Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype.
对秋水仙碱耐药的中国仓鼠卵巢（CHO）细胞突变体，其耐药性源于药物渗透性降低，此前已在我们实验室中分离得到。这种渗透性降低影响多种不相关药物，导致这些突变体呈现多药耐药表型。一种170,000道尔顿的细胞表面糖蛋白（P-糖蛋白）被鉴定出来，其表达似乎与耐药程度相关。在本研究中，我们通过DNA介导的基因转移，将从秋水仙碱耐药突变体获得的DNA导入敏感的小鼠L细胞，使其获得了多药耐药表型。用针对突变CHO细胞膜制备的抗血清进行染色，在这些DNA转化体的质膜中检测到了P-糖蛋白。这些结果与P-糖蛋白表达和多药耐药表型之间的因果关系一致。
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