与哺乳动物细胞系中的多药耐药性相关的细胞表面P-糖蛋白。

Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines.

作者信息

Kartner N, Riordan J R, Ling V

出版信息

Science. 1983 Sep 23;221(4617):1285-8. doi: 10.1126/science.6137059.

Abstract

The plasma membranes of hamster, mouse, and human tumor cell lines that display multiple resistance to drugs were examined by gel electrophoresis and immunoblotting. In every case, increased expression of a 170,000-dalton surface antigen was found to be correlated with multidrug resistance. This membrane component is of identical molecular size and shares some immunogenic homology with the previously characterized P-glycoprotein of colchicine-resistant Chinese hamster ovary cells. This finding may have application to cancer therapy.

摘要

通过凝胶电泳和免疫印迹法对仓鼠、小鼠和人类肿瘤细胞系的质膜进行了检测，这些细胞系对多种药物具有抗性。在每种情况下，均发现一种170,000道尔顿表面抗原的表达增加与多药耐药性相关。这种膜成分具有相同的分子大小，并且与先前鉴定的秋水仙碱抗性中国仓鼠卵巢细胞的P-糖蛋白具有一些免疫原性同源性。这一发现可能在癌症治疗中具有应用价值。