Fliedner T M, Wandl U B, Calvo W
Schweiz Med Wochenschr. 1982 Oct 9;112(41):1423-9.
Autologous transfusion of 2.8-39.5 X 10(9) cryopreserved mononuclear blood leukocytes (BMNC) into 1200 R wholebody irradiated dogs leads within 10 days of irradiation and transfusion to patterns of hemopoietic regeneration of bone marrow, spleen and blood the extent of which correlates with the number of granulocytic progenitor cells, measured as CFU-C, in the tranfusate. In the marrow there are erythrocytic, granulocytic and megakaryocytic foci of regeneration ("colonies") in a proportion of 10:6:1 respectively, while the spleen exhibits cell dose related regeneration of the white pulp and a (transitory) erythropoietic wave of regeneration localized in the red pulp only. The mechanisms of the development of this extramedullary hemopoiesis are unknown. However, it closely resembles the prenatal phase of splenic erythropoiesis which disappears soon after birth. The normoblasts present in the blood at day 10 after irradiation and transfusion of BMNC correlate with extramedullary blood cell formation buy not with the type and extent of bone marrow regeneration.
将2.8 - 39.5×10⁹个冷冻保存的单核血白细胞（BMNC）自体回输给接受1200伦琴全身照射的犬，在照射和输血后10天内，可导致骨髓、脾脏和血液的造血再生模式，其程度与输注物中以CFU - C衡量的粒细胞祖细胞数量相关。在骨髓中，分别有比例为10:6:1的红细胞、粒细胞和巨核细胞再生灶（“集落”），而脾脏则表现出白髓与细胞剂量相关的再生以及仅局限于红髓的（短暂的）红细胞生成再生波。这种髓外造血发展的机制尚不清楚。然而，它与出生后不久就消失的脾脏红细胞生成的产前阶段非常相似。在照射和输注BMNC后第10天血液中出现的幼红细胞与髓外血细胞形成有关，但与骨髓再生的类型和程度无关。
