Schliwa M, Pryzwansky K B, van Blerkom J
Philos Trans R Soc Lond B Biol Sci. 1982 Nov 4;299(1095):199-205. doi: 10.1098/rstb.1982.0126.
The three major filamentous components of the cytoskeleton (microfilaments, microtubules and intermediate filaments) do not just coexist in the cell, but interact with each other in various ways. This paper discusses some examples of structural interactions visualized in critical-point-dried cells by stereoscopic high-voltage electron microscopy. The relative contribution of two classes of interactions to the consolidation of different cytoskeletal domains is considered. One class is represented by T-junctions (end-to-side contacts) of actin filaments with other filaments, and the other by 3 nm links. Attention is then turned to what may be called the behavioural consequences of cytoskeletal interactions. As an example of a coordinated interplay between events at the cell membrane and the cytoskeleton, we discuss changes in cytoskeletal organization of polymorphonuclear leucocytes upon stimulation with a chemotactic factor. These changes culminate in some cells in centriole separation and the establishment of two microtubule asters, each centred around a single centriole.
细胞骨架的三大丝状成分（微丝、微管和中间丝）并非仅仅在细胞中共存，而是以多种方式相互作用。本文讨论了通过立体高压电子显微镜在临界点干燥细胞中观察到的一些结构相互作用的例子。文中考虑了两类相互作用对不同细胞骨架结构域巩固的相对贡献。一类以肌动蛋白丝与其他丝的T形连接（端对侧接触）为代表，另一类以3纳米连接为代表。接着，我们将注意力转向细胞骨架相互作用可能产生的行为后果。作为细胞膜事件与细胞骨架之间协调相互作用的一个例子，我们讨论了趋化因子刺激后多形核白细胞细胞骨架组织的变化。这些变化在一些细胞中最终导致中心粒分离并形成两个微管星状体，每个星状体以单个中心粒为中心。
