分离的和原位的细胞骨架成分的高分辨率扫描电子显微镜观察
High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.
作者信息
Ip W, Fischman D A
出版信息
J Cell Biol. 1979 Oct;83(1):249-54. doi: 10.1083/jcb.83.1.249.
Abstract
Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.
摘要
有证据表明，在使用硫代碳酰肼作为配体对生物材料进行锇浸渍后，通过临界点干燥，利用扫描电子显微镜可以分辨细胞骨架结构（肌动蛋白丝、中间丝和微管）。这些不同类型的丝或管既可以作为纯化的蛋白质聚合物，也可以作为冷冻断裂或去污剂提取细胞内的结构化细胞器来识别。
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