Grozdova M D, Khokhlova Iu V, Panasiuk A F, Guseva N G
Vopr Med Khim. 1982 Nov-Dec;28(6):51-6.
Decrease in content of cAMP and increase--of cGMP as well as stimulation of Ca2+ transport into cells were found in cell cultures of skin fibroblasts from patients with systemic scleroderma (SSD) as compared with normal state. Adrenaline affected dissimilarly the fibroblasts from the patients and healthy donors. In SSD the stimulating effect of adrenaline (10(-7)M) on the cAMP content was decreased more than 6-fold as compared with normal state; at the same time, transport of Ca2+ was inhibited by the hormone (2 X 10(-6)M) in SSD contrary to a stimulating effect of adrenaline in normal cells. Blocking of beta-adrenoreceptors with inderal removed completely the effect of adrenaline on Ca2+ transport both in normal and pathological states. Alteration in the reactions of fibroblasts to catecholamines might be important in deterioration of the physiological mechanism including inhibition of collagen synthesis in SSD.
与正常状态相比，系统性硬化症（SSD）患者皮肤成纤维细胞培养物中发现环磷酸腺苷（cAMP）含量降低，环磷酸鸟苷（cGMP）含量增加，以及钙离子向细胞内转运受到刺激。肾上腺素对患者和健康供体的成纤维细胞影响不同。在SSD中，与正常状态相比，肾上腺素（10⁻⁷M）对cAMP含量的刺激作用降低了6倍以上；与此同时，在SSD中，激素（2×10⁻⁶M）抑制了钙离子转运，这与肾上腺素在正常细胞中的刺激作用相反。用心得安阻断β-肾上腺素能受体完全消除了肾上腺素在正常和病理状态下对钙离子转运的影响。成纤维细胞对儿茶酚胺反应的改变可能在包括SSD中胶原蛋白合成抑制在内的生理机制恶化中起重要作用。
