使用两种抗原性不同的淋球菌菌毛作为抗原的酶联免疫吸附测定法检测淋球菌抗体。
Enzyme-linked immunosorbent assay for detecting gonococcal antibodies using two antigenically different gonococcal pili as antigen.
作者信息
Oranje A P, Reimann K, de Roo A, Schouten H J, Stolz E, Michel M F
出版信息
Br J Vener Dis. 1983 Apr;59(2):89-91. doi: 10.1136/sti.59.2.89.
Antibodies to pilar antigens of two gonococcal strains isolated in Rotterdam (6650 and 1443) were detected using enzyme-linked immunosorbent assays (ELISA). Paired sera (the first sample taken at the first examination (D1) and the second 11-22 days later (D2)) from women with and without gonorrhoea attending a sexually transmitted disease (STD) clinic were studied. The sensitivity of the ELISA using gonococcal pili 6650 as antigen (ELISA 6650) was significantly higher than that using gonococcal pili 1443 as antigen (ELISA 1443). The specificity of the two tests differed little. On D1 the sensitivity in women with uncomplicated gonorrhoea was 69% in the ELISA 6650 and 45% in the ELISA 1443; the corresponding values in asymptomatic infected women were 75% and 57% respectively. The agreement (both in positive and in negative results) between the two tests was less than might have been expected (kappa = 0.41).
采用酶联免疫吸附测定（ELISA）检测在鹿特丹分离出的两株淋球菌菌株（6650和1443）的毛发抗原抗体。研究了性病（STD）门诊有或无淋病的女性的配对血清（第一份样本在首次检查时采集（D1），第二份在11 - 22天后采集（D2））。以淋球菌菌毛6650作为抗原的ELISA（ELISA 6650）的敏感性显著高于以淋球菌菌毛1443作为抗原的ELISA（ELISA 1443）。两种检测的特异性差异不大。在D1时，ELISA 6650中单纯性淋病女性的敏感性为69%，ELISA 1443中为45%；无症状感染女性的相应值分别为75%和57%。两种检测之间的一致性（阳性和阴性结果）低于预期（kappa = 0.41）。
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