Sandberg M, Corazzi L
J Neurochem. 1983 Apr;40(4):917-21. doi: 10.1111/j.1471-4159.1983.tb08074.x.
Tissue slices from the superior colliculi (SC) of the rabbit were superfused and investigated 1 week after unilateral eye removal. Amino acid levels were determined both in the tissue slices and in the medium after chemical depolarisation (56 mM K). The amino acid determinations were done fluorimetrically by precolumn derivation and HPLC separation. Colliculi contralateral to the enucleation exhibited a 16% reduction in glutamate compared with the ipsilateral colliculi. The Ca-dependent release of glutamate or other amino acids tested was not appreciably affected by enucleation. However, both the total and the Ca-independent release of glutamate was lower from contralateral SC slices compared with the ipsilateral slices. The results do not favour glutamate as the major optic nerve transmitter in the rabbit, but do not rule out glutamate as a transmitter in a minor population of retinal fibres.
在单侧眼球摘除1周后，对兔上丘（SC）的组织切片进行了灌流和研究。在化学去极化（56 mM K）后，测定了组织切片和培养基中的氨基酸水平。氨基酸测定通过柱前衍生和高效液相色谱分离进行荧光测定。与摘除眼球同侧的上丘相比，摘除眼球对侧的上丘谷氨酸含量降低了16%。摘除眼球对谷氨酸或其他所检测氨基酸的钙依赖性释放没有明显影响。然而，与同侧切片相比，对侧SC切片中谷氨酸的总释放量和非钙依赖性释放量均较低。这些结果不支持谷氨酸作为兔主要的视神经递质，但也不排除谷氨酸作为少数视网膜纤维中的递质。
