Rossi A M, Migliore L, Barale R, Loprieno N
Teratog Carcinog Mutagen. 1983;3(1):75-87. doi: 10.1002/1520-6866(1990)3:1<75::aid-tcm1770030109>3.0.co;2-5.
In vivo and in vitro methodologies that have employed the yeast Schizosaccharomyces pombe as genetic indicator have been utilized to investigate the mutagenicity of two trichloroethylene (TCE) samples of pure and technical grade. Mutagenicity assays were also performed on two stabilizers contained in the technical grade sample: epichlorohydrin and 1,2-epoxybutane. In the in vitro studies a metabolic conversion system was supplied by liver homogenate (S-9) from mice and rats untreated and pretreated with phenobarbital and/or beta-naphthoflavone. Up to highly toxic doses of TCE were applied to growing and stationary-phase yeast cells. In the in vivo studies two different host-mediated assays, intrasanguineous and intraperitoneal methodologies, were performed on different mice breeds treated by oral administration. Epichlorohydrin and epoxybutane were tested singly or combined in a mixture of the same ratio as in the technical grade TCE sample. Both TCE samples gave negative results for in vivo and in vitro assays, whereas the two contaminants were found mutagenic only in vitro. The high toxicity of the technical TCE sample did not allow us to reach concentrations containing effective levels of its two additives.
已采用粟酒裂殖酵母作为遗传指示物的体内和体外方法，来研究两种纯品和工业级三氯乙烯（TCE）样品的致突变性。还对工业级样品中所含的两种稳定剂——环氧氯丙烷和1,2 - 环氧丁烷进行了致突变性测定。在体外研究中，代谢转化系统由未处理以及经苯巴比妥和/或β - 萘黄酮预处理的小鼠和大鼠的肝脏匀浆（S - 9）提供。对生长阶段和稳定期的酵母细胞施加了高达剧毒剂量的TCE。在体内研究中，对经口服给药处理的不同品系小鼠，采用了两种不同的宿主介导试验方法，即血内注射法和腹腔注射法。环氧氯丙烷和环氧丁烷按照与工业级TCE样品相同的比例单独或混合进行测试。两种TCE样品在体内和体外试验中均给出阴性结果，而这两种污染物仅在体外被发现具有致突变性。工业级TCE样品的高毒性使我们无法达到含有其两种添加剂有效水平的浓度。
