Nakamura S, Kino I, Baba S
Gan. 1983 Feb;74(1):116-21.
Surgically obtained portions of the human gastrointestinal tract were perfused with oxygenated perfluorochemical blood substitute, Fluosol-DA (FDA), containing tritiated thymidine. This procedure of ex vivo autoradiography was performed at the RI Center by perfusion of the isolated organ with FDA from a reservoir 190 cm above the organ through a cannula inserted into the main artery. Microautoradiographic sections revealed that the distributions of labeled nuclei in the normal epithelium of the esophagus, stomach, jejunum, colon and rectum coincided well with those reported previously. However, in the normal colonic mucosa in cases of familial adenomatosis, no expansion or shift of the proliferative zone to the upper part of the mucosa was observed. No significant difference was observed between the labeled figures of normal colonic mucosa of cases of familial adenomatosis and ordinary colonic cancer. This is the first report of ex vivo autoradiography of the gastrointestinal tract of man for studies on cell kinetics.
将取自人体胃肠道的手术标本用含氚化胸腺嘧啶的氧合全氟化学血液代用品氟碳乳剂（Fluosol - DA，美国食品药品监督管理局批准使用）进行灌注。这种离体放射自显影程序是在放射研究中心进行的，通过插入主动脉的插管，将来自高于器官190厘米处储液器的氟碳乳剂灌注到离体器官中。显微放射自显影片显示，食管、胃、空肠、结肠和直肠正常上皮中标记细胞核的分布与先前报道的情况非常吻合。然而，在家族性腺瘤病患者的正常结肠黏膜中，未观察到增殖区向黏膜上部扩展或移位。家族性腺瘤病患者的正常结肠黏膜与普通结肠癌的标记图像之间未观察到显著差异。这是关于人体胃肠道离体放射自显影用于细胞动力学研究的首次报道。
