Weinstein R, Silverblatt F J
J Infect Dis. 1983 May;147(5):882-9. doi: 10.1093/infdis/147.5.882.
To identify mechanisms whereby antibody to mannose-sensitive pili of Escherichia coli might enhance host defenses, we evaluated the activity of antibody to pili in four antibacterial immune processes. Antiserum to pili and Fab' fragments of IgG antibody to pili inhibited the ability of homologous piliated organisms to adhere to buccal epithelial cells. However, this antiserum did not enhance intravascular clearance, complement-dependent bacteriolysis, or opsonophagocytosis. The addition of antiserum to pili to polymorphonuclear leukocytes and piliated E. coli reduced the rate of killing from 52% to 5%. The addition of complement restored the rate to 52%, but this was much less than the 99% rate achieved with polymorphonuclear leukocytes and either fresh serum or antibody to the whole bacteria. These observations suggest that the principal anti-bacterial property of antibody to mannose-sensitive pili of E. coli is inhibition of bacterial attachment. Whether the anti-opsonic effect of antibody to pili is detrimental to host defense remains to be determined.
为了确定抗大肠杆菌甘露糖敏感菌毛抗体增强宿主防御的机制，我们在四个抗菌免疫过程中评估了菌毛抗体的活性。菌毛抗血清和菌毛IgG抗体的Fab'片段抑制了同源菌毛生物体粘附于颊上皮细胞的能力。然而，这种抗血清并未增强血管内清除、补体依赖性细菌溶解或调理吞噬作用。将菌毛抗血清添加到多形核白细胞和菌毛大肠杆菌中，使杀伤率从52%降至5%。添加补体可将杀伤率恢复至52%，但这远低于多形核白细胞与新鲜血清或全菌抗体联合时达到的99%的杀伤率。这些观察结果表明，抗大肠杆菌甘露糖敏感菌毛抗体的主要抗菌特性是抑制细菌附着。菌毛抗体的抗调理作用是否对宿主防御有害仍有待确定。
