Verwoerd D W, Meyer-Scharrer E, Du Plessis J L
Onderstepoort J Vet Res. 1977 Dec;44(4):271-3.
Epithelial cells of the 15.4 line, which were originally established from the adenomatous lung of a jaagsieke case and which had been cultured in vitro for 22 generations, were injected subcutaneously into athymic nude mice. A slow-growing tumour which soon became cystic was established in each case. The cysts rapidly increased in size as a result of the accumulation of a slightly turbid secretion containing aggregates of tumour cells which rapidly refilled the cysts after the fluid had been withdrawn. Cultures were readily re-established from these cells and a chromosomal analysis proved that the tumor consisted of sheep cells. An epithelial cell lining, very similar to that found in the adenomatous lung alveoli of typical jaagsiekte, could be demonstrated histologically.
15.4系上皮细胞最初从一只绵羊肺腺瘤病例的腺瘤性肺组织中分离出来，并在体外培养了22代，然后皮下注射到无胸腺裸鼠体内。每只裸鼠都形成了一个生长缓慢的肿瘤，该肿瘤很快变成囊肿。由于含有肿瘤细胞聚集体的微浊分泌物的积累，囊肿迅速增大，抽出囊液后，肿瘤细胞聚集体又迅速重新充满囊肿。这些细胞很容易重新建立培养物，染色体分析证明该肿瘤由绵羊细胞组成。组织学检查显示，肿瘤的上皮细胞内衬与典型绵羊肺腺瘤病腺瘤性肺泡中的上皮细胞内衬非常相似。
