Mályusz M, Ehrens H J
Enzyme. 1983;29(2):93-9. doi: 10.1159/000469613.
The effect of castration of male rats with experimental renal hypertension ('two-kidney Goldblatt hypertension') was studied on the height of the hypertension and on the urinary output of gamma-glutamyl transpeptidase (gamma GT) and of N-acetyl-beta-D-glucosaminidase (NAG). Castration was carried out immediately after clamping one renal artery. Some of the castrates received testosterone substitution from the 3rd postoperative week onwards. Uncastrated hypertensive males served as controls. The experiments were carried out 8-18 weeks after eliciting high blood pressure. Hypertension as well as enzymuria were less expressed in castrates than in uncastrated males or in testosterone-substituted rats. In all animals studied the gamma GT excretion rate showed a positive correlation with the blood pressure. The output of gamma GT and of NAG as well as the specific gamma GT activity of the renal membrane fraction was lower in castrates than in uncastrated males or in substituted castrates. In uncastrated males and in testosterone-substituted castrates the daily NAG output showed a direct correlation with the renal hydroxyproline content. No such correlation was found in castrated males. The kidneys of castrates and of testosterone-substituted castrates contained less hydroxyproline than those of uncastrated males.
研究了去势对雄性实验性肾性高血压大鼠（“双肾 Goldblatt 高血压”）血压高度以及γ-谷氨酰转肽酶（γGT）和 N-乙酰-β-D-氨基葡萄糖苷酶（NAG）尿排泄量的影响。在夹闭一侧肾动脉后立即进行去势。部分去势大鼠从术后第 3 周起接受睾酮替代治疗。未去势的高血压雄性大鼠作为对照。在引发高血压 8 - 18 周后进行实验。去势大鼠的高血压和酶尿症表现均低于未去势雄性大鼠或接受睾酮替代治疗的大鼠。在所有研究的动物中，γGT 排泄率与血压呈正相关。去势大鼠的γGT 和 NAG 排泄量以及肾膜部分的γGT 比活性均低于未去势雄性大鼠或接受替代治疗的去势大鼠。在未去势雄性大鼠和接受睾酮替代治疗的去势大鼠中，每日 NAG 排泄量与肾羟脯氨酸含量呈直接相关。在去势雄性大鼠中未发现这种相关性。去势大鼠和接受睾酮替代治疗的去势大鼠的肾脏中羟脯氨酸含量低于未去势雄性大鼠。
