前列腺素E2（PGE2）与生长抑素对组胺刺激的离体大鼠胃黏膜细胞摄取14C-氨基比林及环磷酸腺苷（cAMP）生成影响的比较

Comparison of the effect of PGE2 and somatostatin on histamine stimulated 14C-aminopyrine uptake and cyclic AMP formation in isolated rat gastric mucosal cells.

作者信息

Schepp W, Heim H K, Ruoff H J

出版信息

Agents Actions. 1983 Apr;13(2-3):200-6. doi: 10.1007/BF01967331.

DOI:10.1007/BF01967331

Abstract

In isolated rat gastric cells somatostatin and PGE2 were compared in respect to their effects on the cAMP system and on the histamine-stimulated H+-production, measured by 14C-aminopyrine (14C-AP) uptake. Like PGE2 somatostatin activated adenylate cyclase (AC) for all in non-parietal cells. This effect on AC declined in cell fractions with increasing number of parietal cells. Activation of AC or elevation of cellular cAMP and uptake of 14C-AP in response to histamine were inhibited by 10(-9) to 10(-5) mol/1 PGE2 and somatostatin. The results indicate remarkable similarity between somatostatin and PGE2: both activate a non-parietal cell AC and both inhibit H+-production, likely by interfering at the histamine sensitive AC of the parietal cell.

摘要

在分离的大鼠胃细胞中，比较了生长抑素和前列腺素E2（PGE2）对环磷酸腺苷（cAMP）系统以及对组胺刺激的氢离子（H⁺）产生的影响，后者通过¹⁴C - 氨基比林（¹⁴C - AP）摄取来测量。与PGE2一样，生长抑素在所有非壁细胞中均激活腺苷酸环化酶（AC）。随着壁细胞数量增加，这种对AC的作用在细胞组分中减弱。10⁻⁹至10⁻⁵ mol/1的PGE2和生长抑素抑制了AC的激活或细胞内cAMP的升高以及对组胺的¹⁴C - AP摄取。结果表明生长抑素和PGE2之间存在显著相似性：两者均激活非壁细胞AC，并且两者均可能通过干扰壁细胞的组胺敏感AC来抑制H⁺产生。