Jackson S, Stas R J, Potter L M
Poult Sci. 1983 Jun;62(6):1117-9. doi: 10.3382/ps.0621117.
Three experiments were conducted to determine the relative deficiencies of lysine, threonine, valine, and nitrogen per se in a low protein diet composed primarily of corn, soybean meal, and .3% added DL-methionine fed to young turkeys. Diets containing 22, 26, and 30% protein served as controls. A mixture of amino acids (AA) was added to the 22% protein diet. Additional diets were formulated by individually removing lysine, threonine, or valine from the AA mixture and by adding 4% glutamic acid to each to form a 5 X 2 design exclusive of 26 and 30% protein diets. In each experiment, each diet was fed ad libitum to two pens of nine male poults and two pens of nine female poults of a medium body-size variety from 8 to 19 or 20 days of age. Body weight gains and feed consumptions were increased (P less than .001) by supplementing the 22% protein diet with the AA mixture or by increasing the protein level to 26 or 30%. Removal of either lysine, threonine, or valine from the AA mixture produced body weight gains and feed efficiencies smaller (P less than .001) than from the fully supplemented diets but greater (P less than .05) than from the unsupplemented diets. The addition of 4% glutamic acid to the diets failed to improve performance, indicating that nitrogen per se is not the limiting factor for growth. From these results, it is postulated that sulfur AA appear more deficient than either lysine, threonine, or valine in the 22% protein corn-soybean meal diet containing .3% added methionine.
进行了三项试验,以确定在主要由玉米、豆粕和0.3%添加的DL-蛋氨酸组成的低蛋白日粮中,赖氨酸、苏氨酸、缬氨酸和氮本身对幼火鸡的相对缺乏情况。含22%、26%和30%蛋白质的日粮用作对照。向含22%蛋白质的日粮中添加了氨基酸混合物。通过从氨基酸混合物中分别去除赖氨酸、苏氨酸或缬氨酸,并向每种日粮中添加4%的谷氨酸,形成了一个5×2的设计,不包括含26%和30%蛋白质的日粮。在每项试验中,从8日龄至19或20日龄,将每种日粮随意喂给两栏中等体型品种的9只雄性小火鸡和两栏9只雌性小火鸡。通过向含22%蛋白质的日粮中添加氨基酸混合物或将蛋白质水平提高到26%或30%,体重增加和采食量增加(P<0.001)。从氨基酸混合物中去除赖氨酸、苏氨酸或缬氨酸后,体重增加和饲料效率比完全补充的日粮小(P<0.001),但比未补充的日粮大(P<0.05)。向日粮中添加4%的谷氨酸未能改善生长性能,表明氮本身不是生长的限制因素。根据这些结果,推测在添加了0.3%蛋氨酸的含22%蛋白质的玉米-豆粕日粮中,含硫氨基酸比赖氨酸、苏氨酸或缬氨酸更缺乏。