Simonneau M, Tauc L, Baux G
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1661-5. doi: 10.1073/pnas.77.3.1661.
Quantal events have been analyzed at a neuro-neuronal synapse of Aplysia where the nature of the transmitter is established and both presynaptic and postsynaptic neurons are identified and can be voltage-clamped. Prolonged depolarizations were applied to the presynaptic neuron, which gave rise in the postsynaptic cell to a current response characterized by current fluctuations or noise. Acetylcholine was also applied ionophoretically on the same postsynaptic cell. Amplitude and time course of miniature currents and acetylcholine-activated chloride channels of the same cell were examined by using a current fluctuation analysis. It was estimated that one presynaptic spike releases about 180 quanta, each opening 500 chloride channels.
在海兔的神经-神经元突触处对量子事件进行了分析，在该突触处已确定了递质的性质，并且已识别出突触前和突触后神经元，并且可以对它们进行电压钳制。对突触前神经元施加长时间的去极化，这在突触后细胞中产生了以电流波动或噪声为特征的电流响应。还通过离子电泳法将乙酰胆碱施加到同一个突触后细胞上。通过电流波动分析检查了同一个细胞的微小电流和乙酰胆碱激活的氯离子通道的幅度和时间进程。据估计，一个突触前尖峰释放约180个量子，每个量子打开500个氯离子通道。
