Briggs M H
Int J Fertil. 1983;28(2):107-9.
Groups of 10 pregnant albino rats received a single subcutaneous injection of oil containing synthetic sex hormones on day 13 of pregnancy. Control rats received only oil. The steroid doses were ten times those used as oral hormonal pregnancy tests for humans. Norethisterone acetate (NEA) was given at 2 mg/kg, ethynylestradiol (EE) at 4 micrograms/kg, and NEA + EE together at these same doses. The concentration of penile total specific androgen-receptor binding sites in cytosol preparations was measured by means of in vitro uptake of tritiated methyltrienolone (3H-R 1881). No significant difference was observed between controls and treated groups for androgen-receptor concentrations in preparations from male offspring at birth, or in others reared to day 30. Rat penile androgen receptors showed high affinity for 5 alpha-dihydrotestosterone. Testosterone showed weaker binding (ca. 10%), while R 1881 showed much greater affinity (ca. 550%). Very weak binding was shown by progesterone, estradiol 17 beta, and the synthetic steroids investigated in this study.
将10只怀孕的白化病大鼠分为一组，在妊娠第13天接受一次皮下注射含合成性激素的油剂。对照大鼠仅接受油剂。类固醇剂量是用于人类口服激素妊娠试验剂量的10倍。醋酸炔诺酮（NEA）以2毫克/千克给药，炔雌醇（EE）以4微克/千克给药，NEA和EE以相同剂量联合给药。通过氚标记的甲基三烯olone（3H-R 1881）的体外摄取来测量细胞溶质制剂中阴茎总特异性雄激素受体结合位点的浓度。在出生时雄性后代的制剂中，或在饲养至第30天的其他后代中，对照组和治疗组之间的雄激素受体浓度没有观察到显著差异。大鼠阴茎雄激素受体对5α-二氢睾酮表现出高亲和力。睾酮表现出较弱的结合（约10%），而R 1881表现出更大的亲和力（约550%）。孕酮、17β-雌二醇和本研究中研究的合成类固醇表现出非常弱的结合。
