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通过时间分辨磷光去极化测量肌质网Ca2+转运三磷酸腺苷酶的节段运动和旋转扩散。

Segmental motion and rotational diffusion of the Ca2+-translocating adenosine triphosphatase of sarcoplasmic reticulum, measured by time-resolved phosphorescence depolarization.

作者信息

Speirs A, Moore C H, Boxer D H, Garland P B

出版信息

Biochem J. 1983 Jul 1;213(1):67-74. doi: 10.1042/bj2130067.

DOI:10.1042/bj2130067
PMID:6137210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152091/
Abstract

We studied the rotational mobility of the Ca2+ + Mg2+-activated ATPase in skeletal-muscle sarcoplasmic-reticulum vesicles, using time-resolved measurements of the depolarization of laser-flash-excited phosphorescence of the extrinsic triplet probe erythrosin. Our results are in general agreement with those of others [Bürkli & Cherry (1981) Biochemistry 20, 138-145] obtained by linear dichroism methods. In addition, we directly observed fast depolarization in the 1-5 microseconds time range that can be attributed to limited motion of part of the protein (segmental motion). Temperature-dependent changes in phosphorescence anisotropy indicated the onset of a conformational change in structure of the Ca2+ + Mg2+-activated ATPase at 11-13 degrees C. We also describe the synthesis of 5-iodoacetamidoerythrosin.

摘要

我们利用对外部三重态探针赤藓红激光闪光激发磷光去极化的时间分辨测量，研究了骨骼肌肌浆网囊泡中Ca2+ + Mg2+激活的ATP酶的旋转流动性。我们的结果与其他人[布尔克利和切里（1981年），《生物化学》20卷，第138 - 145页]通过线性二色性方法得到的结果总体一致。此外，我们直接观察到在1 - 5微秒时间范围内的快速去极化，这可归因于蛋白质部分区域的有限运动（片段运动）。磷光各向异性的温度依赖性变化表明，在11 - 13摄氏度时，Ca2+ + Mg2+激活的ATP酶结构发生了构象变化。我们还描述了5 - 碘乙酰氨基赤藓红的合成。

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Biophys J. 1986 Jul;50(1):41-53. doi: 10.1016/S0006-3495(86)83437-3.
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Applications of new saturation transfer electron paramagnetic resonance methodology to the rotational dynamics of the Ca-ATPase in sarcoplasmic reticulum membranes.新型饱和转移电子顺磁共振方法在肌浆网膜中钙 - ATP酶旋转动力学研究中的应用。
Biophys J. 1986 Apr;49(4):937-42. doi: 10.1016/S0006-3495(86)83721-3.
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Fluorescence energy transfer as an indicator of Ca2+-ATPase interactions in sarcoplasmic reticulum.荧光能量转移作为肌浆网中Ca2 + -ATP酶相互作用的指标。
Biophys J. 1987 Feb;51(2):205-20. doi: 10.1016/S0006-3495(87)83326-X.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Rotational mobility of the membrane-bound acetylcholine receptor of Torpedo electric organ measured by phosphorescence depolarisation.用磷光去极化法测量电鳐电器官中膜结合型乙酰胆碱受体的旋转流动性。
FEBS Lett. 1980 Mar 10;111(2):407-12. doi: 10.1016/0014-5793(80)80838-6.
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Rotational diffusion of biological macromolecules by time-resolved delayed luminescence (phosphorescence, fluorescence) anisotropy.通过时间分辨延迟发光（磷光、荧光）各向异性研究生物大分子的旋转扩散。
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Laser Raman characterization of conformational changes in sarcoplasmic reticulum induced by temperature, Ca2+, and Mg2+.
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Rotational dynamics of protein and boundary lipid in sarcoplasmic reticulum membrane.肌质网膜中蛋白质与边界脂质的旋转动力学
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Rotational diffusion of epidermal growth factor complexed to cell surface receptors reflects rapid microaggregation and endocytosis of occupied receptors.与细胞表面受体复合的表皮生长因子的旋转扩散反映了被占据受体的快速微聚集和内吞作用。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6981-5. doi: 10.1073/pnas.78.11.6981.

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