pH对芳基硫酸酯酶A和B动力学的影响。
The effect of pH on the kinetics of arylsulphatases A and B.
作者信息
O'Fagain C, Butler B M, Mantle T J
出版信息
Biochem J. 1983 Sep 1;213(3):603-7. doi: 10.1042/bj2130603.
Abstract
The effect of pH on the kinetics of rat liver arylsulphatases A and B is very similar and shows that two groups with pK values of 4.4-4.5 and 5.7-5.8 are important for enzyme activity. Substrate binding has no effect on the group with a pK of 4.4-4.5; however, the pK of the second group is shifted to 7.1-7.5 in the enzyme-substrate complex. An analysis of the effect of pH on the Ki for sulphate inhibition suggests that HSO4-is the true product. A model is proposed that involves the two ionizing groups identified in the present study in a concerted general acid-base-catalysed mechanism.
摘要
pH对大鼠肝脏芳基硫酸酯酶A和B动力学的影响非常相似，表明两个pK值分别为4.4 - 4.5和5.7 - 5.8的基团对酶活性很重要。底物结合对pK为4.4 - 4.5的基团没有影响；然而，在酶 - 底物复合物中，第二个基团的pK值移至7.1 - 7.5。对pH对硫酸盐抑制的Ki影响的分析表明，HSO4-是真正的产物。提出了一个模型，该模型在协同的一般酸碱催化机制中涉及本研究中鉴定的两个电离基团。
相似文献
1
The effect of pH on the kinetics of arylsulphatases A and B.pH对芳基硫酸酯酶A和B动力学的影响。
Biochem J. 1983 Sep 1;213(3):603-7. doi: 10.1042/bj2130603.
2
Comparative activity of sulphatases in human liver on two synthetic substrates.人肝脏中硫酸酯酶对两种合成底物的比较活性。
Clin Chim Acta. 1977 Nov 1;80(3):423-9. doi: 10.1016/0009-8981(77)90134-6.
3
Arylsulphatases A and B in the oviduct of female rabbits in anestrus and estrus conditions.处于发情间期和发情期的雌性兔输卵管中的芳基硫酸酯酶A和B
Acta Histochem. 1984;75(2):141-8. doi: 10.1016/S0065-1281(84)80050-1.
4
Localization of the activity of arylsulphatases A and B in the rat salivary glands.大鼠唾液腺中芳基硫酸酯酶A和B活性的定位
Acta Histochem. 1981;69(1):70-6. doi: 10.1016/S0065-1281(81)80011-6.
5
Soluble arylsulphatases in the rabbit salivary glands. A light and electron microscopy study.兔唾液腺中的可溶性芳基硫酸酯酶。光镜和电镜研究。
Acta Histochem. 1983;73(2):193-203. doi: 10.1016/S0065-1281(83)80028-2.
6
Comparative studies of rodent anionic arylsulfatases.啮齿动物阴离子芳基硫酸酯酶的比较研究。
Comp Biochem Physiol B. 1985;82(1):55-61. doi: 10.1016/0305-0491(85)90127-0.
7
Biochemical variability of arylsulphatases -A, -B and -C in cultured fibroblasts from patients with multiple sulphatase deficiency.多种硫酸酯酶缺乏症患者培养成纤维细胞中芳基硫酸酯酶A、B和C的生化变异性
J Inherit Metab Dis. 1983;6(4):167-72. doi: 10.1007/BF02310875.
8
Mammalian arylsulfatases A and B: relative rates of hydrolysis of artificial substrates.哺乳动物芳基硫酸酯酶A和B：人工底物的相对水解速率。
Experientia. 1986 Feb 15;42(2):150-1. doi: 10.1007/BF01952440.
9
Effects of acute and chronic administration of ethanol on rat brain arylsulphatase A and B.急性和慢性给予乙醇对大鼠脑芳基硫酸酯酶A和B的影响。
Life Sci. 1980 Nov 10;27(19):1777-82. doi: 10.1016/0024-3205(80)90445-2.
10
Fluctuations in arylsulphatase activity in the rabbit endometrium during the sexual cycle.家兔性周期中子宫内膜芳基硫酸酯酶活性的波动
Histochemistry. 1985;83(2):127-31. doi: 10.1007/BF00495142.
引用本文的文献
1
Kinetic consequences of the endogenous ligand to molybdenum in the DMSO reductase family: a case study with periplasmic nitrate reductase.内源性配体对 DMSO 还原酶家族中钼的动力学影响：以周质硝酸盐还原酶为例的研究。
J Biol Inorg Chem. 2021 Feb;26(1):13-28. doi: 10.1007/s00775-020-01833-9. Epub 2020 Nov 1.
2
The anomalous kinetics of sulphatase A.硫酸酯酶A的异常动力学
Biochem J. 1989 Aug 1;261(3):689-97. doi: 10.1042/bj2610689.
3
Aluminofluorides and beryllofluorides as inhibitors of sulphatases. Analogues of hydrogen sulphate?作为硫酸酯酶抑制剂的铝氟化物和铍氟化物。硫酸氢盐的类似物？
Biochem J. 1991 Oct 15;279 ( Pt 2)(Pt 2):361-5. doi: 10.1042/bj2790361.
本文引用的文献
1
Statistical estimations in enzyme kinetics.酶动力学中的统计估计
Biochem J. 1961 Aug;80(2):324-32. doi: 10.1042/bj0800324.
2
Studies on sulphatases. 20. Enzymic cleavage of aryl hydrogen sulphates in the presence of H218O.硫酸酯酶研究。20. 在H218O存在下芳基硫酸氢酯的酶促裂解。
Biochem J. 1958 May;69(1):155-9. doi: 10.1042/bj0690155.
3
Studies on sulphatases. I. The choice of substrate for the assay of rat-liver arylsulphatase.硫酸酯酶的研究。I. 大鼠肝脏芳基硫酸酯酶测定底物的选择。
Biochem J. 1953 Feb;53(3):444-51. doi: 10.1042/bj0530444.
4
Purification and properties of human N-acetylgalactosamine-6-sulfate sulfatase.人N-乙酰半乳糖胺-6-硫酸酯硫酸酯酶的纯化及性质
Biochim Biophys Acta. 1981 Feb 13;657(2):344-55. doi: 10.1016/0005-2744(81)90320-x.
5
A glucosamine O,N-disulfate O-sulfohydrolase with a probable role in mammalian catabolism of heparan sulfate.一种可能在哺乳动物硫酸乙酰肝素分解代谢中起作用的氨基葡萄糖O,N-二硫酸O-硫酸水解酶。
Biochem Biophys Res Commun. 1980 Nov 28;97(2):827-33. doi: 10.1016/0006-291x(80)90338-1.
6
The slow kinetic transients of arylsulphatase A.芳基硫酸酯酶A的缓慢动力学瞬变
Biochem J. 1982 Feb 1;201(2):345-52. doi: 10.1042/bj2010345.
7
Inhibition of arylsulphatases A and B by nucleotides and other anions.核苷酸及其他阴离子对芳基硫酸酯酶A和B的抑制作用。
Biochem Soc Trans. 1980 Aug;8(4):450-1. doi: 10.1042/bst0080450.
8
Cerebroside 3-sulfate as a physiological substrate of arylsulfatase A.硫酸脑苷脂作为芳基硫酸酯酶A的生理底物。
Biochim Biophys Acta. 1968 Mar 25;151(3):619-27. doi: 10.1016/0005-2744(68)90008-9.
9
Sulfoglycerogalactolipid from rat testis: a substrate for pure human arylsulfatase A.来自大鼠睾丸的硫代甘油半乳糖脂：纯人芳基硫酸酯酶A的一种底物。
Biochem Biophys Res Commun. 1974 Nov 6;61(1):348-54. doi: 10.1016/0006-291x(74)90573-7.
10
Purification of arylsulfatase A from boar testis and its activities toward seminolipid and sulfatide.从公猪睾丸中纯化芳基硫酸酯酶A及其对半乳糖脑苷脂硫酸酯和硫脂的活性
J Biochem. 1974 Jun;75(6):1241-7. doi: 10.1093/oxfordjournals.jbchem.a130507.
Zotero 插件