Bethlenfalvay N C, Waterman M R, Lima J E, Waldrup T
Comp Biochem Physiol A Comp Physiol. 1983;75(4):635-9. doi: 10.1016/0300-9629(83)90433-4.
Glucose-depleted, nitrite-treated opossum erythrocytes effectively reduce methemoglobin in an environment of physiological saline and added glucose does not accelerate the rate of reduction. In autologous plasma or 25 mM phosphate-buffered saline pH 7.4, added glucose significantly accelerates methemoglobin reduction in glucose-depleted, nitrite-treated opossum erythrocytes. Human red cells require added glucose to carry out reduction of methemoglobin and increased phosphate concentration or autologous plasma does not alter the rate of this process. Within the opossum red cell in vitro, autooxidation of hemoglobin proceeds at a much slower rate than that observed in human erythrocytes.
葡萄糖耗尽、经亚硝酸盐处理的负鼠红细胞在生理盐水环境中能有效还原高铁血红蛋白，添加葡萄糖并不会加快还原速率。在自体血浆或pH 7.4的25 mM磷酸盐缓冲盐水中，添加葡萄糖能显著加快葡萄糖耗尽、经亚硝酸盐处理的负鼠红细胞中高铁血红蛋白的还原。人类红细胞需要添加葡萄糖才能还原高铁血红蛋白，增加磷酸盐浓度或自体血浆并不会改变这一过程的速率。在体外负鼠红细胞内，血红蛋白的自动氧化速率比在人类红细胞中观察到的要慢得多。
