Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--III. Metabolic depletion with 2-deoxyglucose markedly accelerates methemoglobin reduction in opossum but not in human erythrocytes.
作者信息
Bethlenfalvay N C, Lima J E, Chadwick E, Stewart I
机构信息
Department of Primary Care, Fitzsimons Army Medical Center, Aurora, CO 80045-5000.
出版信息
Comp Biochem Physiol A Comp Physiol. 1988;89(2):119-24. doi: 10.1016/0300-9629(88)91067-5.
Glucose-depleted, nitrite-treated erythrocytes reduce ferriheme in vitro in an environment 100 mM to 2-deoxy-D-glucose at a rate of 2.4 microM/ml cells/hr (opossum) and 0.37 microM/ml cells/hr (human). 2. During the process of methemoglobin reduction the breakdown of adenine ribonucleotides is more rapid in opossum (0.9 microM/g hg/hr) than in human (0.36 microM/g hg/hr) erythrocytes. 3. Radiolabelled ribose from [U-14C] ATP is catabolized exclusively to [14C] lactate in opossum, and to [14C] pyruvate and [14C] lactate in human red cells.
摘要
葡萄糖耗尽、经亚硝酸盐处理的红细胞在含有100 mM 2-脱氧-D-葡萄糖的体外环境中，以2.4微摩尔/毫升细胞/小时（负鼠）和0.37微摩尔/毫升细胞/小时（人类）的速率还原高铁血红素。2. 在高铁血红蛋白还原过程中，负鼠红细胞（0.9微摩尔/克血红蛋白/小时）中腺嘌呤核糖核苷酸的分解比人类红细胞（0.36微摩尔/克血红蛋白/小时）更快。3. [U-14C]ATP中的放射性标记核糖在负鼠红细胞中仅分解为[14C]乳酸，而在人类红细胞中分解为[14C]丙酮酸和[14C]乳酸。
