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负鼠（弗吉尼亚负鼠）红细胞能量代谢的研究——II. 负鼠和人类红细胞体外2-脱氧-D-葡萄糖分解代谢的比较方面

Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--II. Comparative aspects of 2-deoxy-D-glucose catabolism in opossum and human red cells in vitro.

作者信息

Bethlenfalvay N C, Lima J E, Waldrup T L, Chadwick E, Stewart I

机构信息

Department of Primary Care, Fitzsimons Army Medical Center, Aurora, CO 80045-5000.

出版信息

Comp Biochem Physiol A Comp Physiol. 1988;89(2):113-7. doi: 10.1016/0300-9629(88)91066-3.

DOI:10.1016/0300-9629(88)91066-3
PMID:2896089
Abstract
  1. G-6-P, F-6-P, F-1, 6-P, DHAP and GA-3-P in opossum erythrocytes were found at levels above those reported in human red cells. 2. About 1% of the radioactivity provided as [1-14C] DOG to red cells of both species was recovered as 14CO2 in 1 hr. 3. Unlike [1-14C] DOG, radiochromatography of extracts of cells incubated DOG revealed two diffusible radiolabelled compounds in the supernatant of cell suspensions. 4. The catabolism of DOG was quantitatively and qualitatively similar in opossum and human erythrocytes under the conditions of this study.
摘要
  1. 负鼠红细胞中的葡萄糖-6-磷酸、果糖-6-磷酸、果糖-1,6-二磷酸、磷酸二羟丙酮和3-磷酸甘油醛的含量高于人类红细胞中报道的水平。2. 两种物种的红细胞在1小时内，以[1-¹⁴C]葡萄糖作为放射性来源，约1%的放射性以¹⁴CO₂的形式回收。3. 与[1-¹⁴C]葡萄糖不同，用葡萄糖孵育的细胞提取物的放射色谱显示，细胞悬液上清中有两种可扩散的放射性标记化合物。4. 在本研究条件下，葡萄糖在负鼠和人类红细胞中的分解代谢在数量和质量上相似。

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1
Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--II. Comparative aspects of 2-deoxy-D-glucose catabolism in opossum and human red cells in vitro.负鼠（弗吉尼亚负鼠）红细胞能量代谢的研究——II. 负鼠和人类红细胞体外2-脱氧-D-葡萄糖分解代谢的比较方面
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Studies on the energy metabolism of opossum Didelphis virginiana erythrocytes--IV. Red cells have low adenosine deaminase activity and high levels of deoxyadenosine nucleotides.负鼠弗吉尼亚负鼠红细胞能量代谢的研究——IV. 红细胞腺苷脱氨酶活性低，脱氧腺苷核苷酸水平高。
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