Reid G, Brooks H J, Bacon D F
J Infect Dis. 1983 Sep;148(3):412-21. doi: 10.1093/infdis/148.3.412.
An in vitro system was used for assay of the attachment of Escherichia coli to viable uroepithelial cells harvested from the urine of premenopausal women. A 15% level of variation from the mean attachment value was noted within given cell samples. Uroepithelial target cells with large numbers of adherent bacteria were found. The receptivity of uroepithelial cells to the attachment of certain strains of E coli varied over the menstrual cycle and during pregnancy. This finding may suggest that adult women are more susceptible to urinary tract infection at particular times during their reproductive period. Double-staining techniques were used for the detection of bacterial attachment to mucopolysaccharide-coated and non-mucopolysaccharide-coated uroepithelial cells. The level of attachment of one strain of E coli was higher when cells were mucus coated, while the attachment of a second strain was unaffected by the presence of a mucus coat. The results suggest the existence of at least two different mechanisms whereby E coli attaches to uroepithelial cells.
采用体外系统检测大肠杆菌与从绝经前女性尿液中获取的活尿路上皮细胞的黏附情况。在给定的细胞样本中，观察到黏附平均值有15%的变化水平。发现了带有大量黏附细菌的尿路上皮靶细胞。尿路上皮细胞对某些大肠杆菌菌株黏附的接受能力在月经周期和孕期有所不同。这一发现可能表明成年女性在其生殖期的特定时间更容易发生尿路感染。采用双重染色技术检测细菌与黏多糖包被和未被黏多糖包被的尿路上皮细胞的黏附情况。当细胞被黏液包被时，一种大肠杆菌菌株的黏附水平较高，而另一种菌株的黏附则不受黏液包被的影响。结果表明至少存在两种不同的机制，大肠杆菌借此黏附于尿路上皮细胞。
