Millsap K, Reid G, van der Mei H C, Busscher H J
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
Appl Environ Microbiol. 1994 Jun;60(6):1867-74. doi: 10.1128/aem.60.6.1867-1874.1994.
The displacement of Enterococcus faecalis 1131 from hydrophobic and hydrophilic substrata by isolates of Lactobacillus casei 36 and Streptococcus hyointestinalis KM1 was studied in a parallel plate flow chamber. The experiments were conducted with either 10 mM potassium phosphate buffer or human urine as the suspending fluid, and adhesion and displacement were measured by real-time in situ image analysis. The results showed that E. faecalis 1131 was displaced by lactobacilli (31%) and streptococci (74%) from fluorinated ethylene propylene in buffer and that displacement by lactobacilli was even more effective on a glass substratum in urine (54%). The passage of an air-liquid interface significantly impacted on adhesion, especially when the surface had been challenged with lactobacilli (up to 100% displacement) or streptococci (up to 94% displacement). These results showed that the parallel plate flow system with real-time in situ image analysis was effective for studying bacterial adhesion and that uropathogenic enterococci can be displaced by indigenous bacteria.
在平行板流动腔中研究了干酪乳杆菌36株和猪肠道链球菌KM1株对粪肠球菌1131从疏水和亲水基质上的取代作用。实验以10 mM磷酸钾缓冲液或人尿液作为悬浮液进行，通过实时原位图像分析测量黏附力和取代作用。结果表明，在缓冲液中，粪肠球菌1131被乳杆菌（31%）和链球菌（74%）从氟化乙丙烯上取代，而在尿液中的玻璃基质上，乳杆菌的取代作用更有效（54%）。气液界面的通过对黏附力有显著影响，尤其是当表面受到乳杆菌（高达100%取代）或链球菌（高达94%取代）挑战时。这些结果表明，具有实时原位图像分析的平行板流动系统对于研究细菌黏附是有效的，并且尿路致病性肠球菌可被本土细菌取代。
