血管活性肠肽瘤患者腹泻与第二种活性肽（肽组氨酸异亮氨酸）的共同分泌有关，这由单一编码基因解释。

Diarrhoea in vipoma patients associated with cosecretion of a second active peptide (peptide histidine isoleucine) explained by single coding gene.

作者信息

Bloom S R, Christofides N D, Delamarter J, Buell G, Kawashima E, Polak J M

出版信息

Lancet. 1983 Nov 19;2(8360):1163-5. doi: 10.1016/s0140-6736(83)91215-1.

Abstract

Peptide histidine isoleucine (PHI), first isolated from pig intestine, is distributed identically to vasoactive intestinal peptide (VIP) in all mammals. 42 patients with high plasma VIP secondary to VIPoma also had very high plasma PHI-like immunoreactivity, in a constant ratio to VIP. None of 125 patients with other endocrine tumours had high levels of either peptide. VIPoma tissue from 20 patients also contained PHI shown by immunocytochemistry to be produced by the same cell as VIP. Messenger RNA(mRNA) from one of these tumours contained the codes for VIP and a separate PHI-like sequence. Human PHI-like sequence differed from porcine PHI in only two aminoacid residues. A single cell thus produces two separate regulatory peptides with apparently similar potencies but different spectra of activity. In normal tissue the constant coproduction of two active neuropeptides by a single neuron provides further evidence against the doctrine of one neuron producing only one neurotransmitter.

摘要

肽组氨酸异亮氨酸（PHI）最初是从猪肠道中分离出来的，在所有哺乳动物中其分布与血管活性肠肽（VIP）相同。42例因VIP瘤导致血浆VIP升高的患者，其血浆PHI样免疫反应性也非常高，且与VIP呈固定比例。125例患有其他内分泌肿瘤的患者中，没有一例两种肽的水平升高。20例患者的VIP瘤组织也含有PHI，免疫细胞化学显示其与VIP由同一细胞产生。其中一个肿瘤的信使核糖核酸（mRNA）包含VIP编码和一个单独的PHI样序列。人类PHI样序列与猪PHI仅在两个氨基酸残基上不同。因此，单个细胞产生两种单独的调节肽，它们的效力明显相似，但活性谱不同。在正常组织中，单个神经元持续共同产生两种活性神经肽，这进一步证明了一个神经元只产生一种神经递质这一学说的错误。