Sorenson G D, Pettengill O S, Cate C C, Ghatei M A, Molyneux K E, Gosselin E J, Bloom S R
Life Sci. 1983 Nov 7;33(19):1939-44. doi: 10.1016/0024-3205(83)90679-3.
Three established cell lines derived from human small cell carcinoma of the lung, and known to produce significant amounts of peptide hormones were used to evaluate the regulation of hormone secretion by cholinergic agonists. In two of the cell lines (DMS 53, DMS 153) acetylcholine chloride, bethanechol chloride, and carbamylcholine at the concentrations of 10(-3)M to 10(-5)M stimulated secretion of bombesin and calcitonin as measured by RIA. The third cell line, DMS 406, was not significantly stimulated. Inhibition of induced stimulation by the cholinergic antagonist atropine, but not hexamethonium, indicated the presence of muscarinic rather than the nicotinic type of cholinergic receptors on the stimulatable cells. These receptors appear to mediate hormone secretion comparably to normal endocrine cells.
三种源自人肺小细胞癌的成熟细胞系，已知它们能产生大量肽类激素，被用于评估胆碱能激动剂对激素分泌的调节作用。在其中两个细胞系（DMS 53、DMS 153）中，浓度为10(-3)M至10(-5)M的氯化乙酰胆碱、氯化贝胆碱和氨甲酰胆碱，通过放射免疫分析测定，刺激了蛙皮素和降钙素的分泌。第三个细胞系DMS 406未受到显著刺激。胆碱能拮抗剂阿托品而非六甲铵对诱导刺激的抑制作用，表明可刺激细胞上存在毒蕈碱型而非烟碱型胆碱能受体。这些受体在介导激素分泌方面似乎与正常内分泌细胞相当。
