Camplejohn R S, Nias A H
Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;44(2):163-71. doi: 10.1007/BF02890167.
Diurnal changes in proliferative activity were investigated in tumour and small intestinal epithelium of mice bearing a transplanted mammary carcinoma. In addition to mitotic and labelling index studies, the metaphase-arrest technique with vincristine (VCR) was employed. In the tumour there was no clear evidence of a significant diurnal rhythm in proliferative activity but in the small intestinal epithelium such a rhythm was clearly demonstrated. A higher cell production rate (kB) measured by metaphase-arrest and higher labelling and mitotic indices were seen in the mid to late part of the dark period. The peak mitotic index was seen 3 to 6 h after the labelling peak in the small intestine. The basal third of the crypt which is believed to include the stem cell compartment of this tissue showed larger diurnal fluctuations in both labelling index and kB than the rest of the proliferative compartment.
对患有移植性乳腺癌的小鼠的肿瘤和小肠上皮中的增殖活性的昼夜变化进行了研究。除了进行有丝分裂和标记指数研究外，还采用了长春新碱（VCR）中期阻断技术。在肿瘤中，没有明显证据表明增殖活性存在显著的昼夜节律，但在小肠上皮中，这种节律得到了明确证实。在黑暗期的中后期，通过中期阻断测量的细胞产生率（kB）更高，标记和有丝分裂指数也更高。在小肠中，有丝分裂指数峰值出现在标记峰值后3至6小时。隐窝的基部三分之一被认为包含该组织的干细胞区室，其标记指数和kB的昼夜波动比增殖区室的其余部分更大。
