Yamada E, Hazama F, Kataoka H, Amano S, Sasahara M, Kayembe K, Katayama K
Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;44(2):241-5. doi: 10.1007/BF02890173.
In an attempt to obtain information regarding vascular elastase in arterial hypertension, we examined biochemical changes in elastase-like enzyme activity, and the intravascular localization of elastase by immunohistochemical techniques in the aorta of spontaneously hypertensive rats (SHR). In the biochemical study, aortic elastase-like enzyme activity was significantly higher in SHR than in controls. Using an antibody against rat pancreatic elastase raised in the rabbit, it was demonstrated immunohistochemically that the enzyme was localized in the endothelial cells and subendothelial spaces in the aorta of control animals. In SHR, elastase was also demonstrated in medial smooth muscle cells and particularly in the modified smooth muscle cells in areas of intimal thickening. Some vacuoles in the smooth muscle cells also showed positive enzyme staining. Elastase seems to play an important role in the development of hypertensive vascular changes.
为了获取有关动脉高血压中血管弹性蛋白酶的信息，我们通过免疫组织化学技术研究了自发性高血压大鼠（SHR）主动脉中弹性蛋白酶样酶活性的生化变化以及弹性蛋白酶的血管内定位。在生化研究中，SHR主动脉中的弹性蛋白酶样酶活性显著高于对照组。使用兔抗大鼠胰腺弹性蛋白酶抗体，免疫组织化学显示该酶定位于对照动物主动脉的内皮细胞和内皮下间隙。在SHR中，弹性蛋白酶也在内膜平滑肌细胞中被检测到，尤其是在内膜增厚区域的变形平滑肌细胞中。平滑肌细胞中的一些空泡也显示出阳性酶染色。弹性蛋白酶似乎在高血压血管变化的发展中起重要作用。
