一种用于 Thy-1 抗原的无细胞可溶性相放射免疫测定法。
A cell-free soluble-phase radioimmunoassay for Thy-1 antigen.
作者信息
Shalev A, Zuckerman F
出版信息
Immunology. 1983 Dec;50(4):667-70.
A cell-free, soluble-phase, radioimmunoassay has been developed for Thy-1 antigen. The method is based on immunoprecipitation of radiolabelled Thy-1 molecules with specific antibodies, anti-immunoglobulin serum and polyethyleneglycol (PEG). The method can be used with convenience to screen for the presence of Thy-1 in various fluids as well as on cell surfaces for qualitative or quantitative purposes. Presence of antibodies or autoantibodies against Thy-1 can also be detected specifically. Evidence that the dog, carp, hamster and goldfish carry Thy-1-like molecules on neuronal (brain) cells is demonstrated by this method.
已开发出一种用于Thy-1抗原的无细胞、可溶性阶段放射免疫测定法。该方法基于用特异性抗体、抗免疫球蛋白血清和聚乙二醇（PEG）对放射性标记的Thy-1分子进行免疫沉淀。该方法可方便地用于筛查各种体液以及细胞表面是否存在Thy-1，以进行定性或定量分析。也可特异性检测针对Thy-1的抗体或自身抗体的存在。该方法证明了狗、鲤鱼、仓鼠和金鱼的神经（脑）细胞上携带Thy-1样分子。
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