Mansour M H, Cooper E L
Eur J Immunol. 1984 Nov;14(11):1031-9. doi: 10.1002/eji.1830141113.
The occurrence of a Thy-1 homolog in the tissues of the advanced invertebrate group of tunicates has been investigated by using two monoclonal antibodies specific to rodent Thy-1.1 determinants and a heterologous anti-rat Thy-1 antiserum. In cellular and soluble-phase radioimmunoassays for Thy-1 using intact hemocytes, tissue extracts and hemolymph, membrane-bound and soluble Thy-1.1 cross-reacting determinants have been detected. Immunoprecipitation experiments with hemocyte deoxycholate extracts and hemolymph, labeled with 125I by lactoperoxidase/chloramine T and with NaB3H4 by galactose oxidase, established the association of the Thy-1.1 cross-reacting determinants with a single glycoprotein of apparent m.w. estimated at 27 000 in hemocytes and 22 500 in hemolymph by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The significance of this finding is discussed in relation to the phylogenetic emergence of the Thy-1 molecule during evolution.
利用两种对啮齿动物Thy-1.1决定簇具有特异性的单克隆抗体以及一种异源抗大鼠Thy-1抗血清，研究了被囊动物这一高等无脊椎动物组织中Thy-1同源物的存在情况。在使用完整血细胞、组织提取物和血淋巴进行的Thy-1细胞和可溶性相放射免疫测定中，已检测到膜结合和可溶性的Thy-1.1交叉反应决定簇。用经乳过氧化物酶/氯胺T标记125I以及经半乳糖氧化酶标记NaB3H4的血细胞脱氧胆酸盐提取物和血淋巴进行免疫沉淀实验，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳确定，Thy-1.1交叉反应决定簇与一种表观分子量的单一糖蛋白相关，该糖蛋白在血细胞中的表观分子量估计为27000，在血淋巴中的表观分子量估计为22500。结合Thy-1分子在进化过程中的系统发育出现情况，对这一发现的意义进行了讨论。
