Prasad L B, Spradbrow P B
Aust Vet J. 1977 Dec;53(12):582-5. doi: 10.1111/j.1751-0813.1977.tb15831.x.
Turkey herpesvirus (HVT) was isolated from the kidneys of 6 of 8 turkey poults from two flocks. The isolates were identified by syncytial-type of cytopathology, inhibition of plaque formation by 5-bromodeoxyuridine, formation of Cowdry type A intranuclear inclusions in cell cultures, and presence of herpes-type virions in negatively stained preparations and thin sections of infected cell cultures. One of these isolates inoculated into chickens proved apathogenic over an observation period of 10 weeks. Indirect immunofluorescence and serum neutralisation tests revealed serological relationship between these isolates and the strain NSW 1/70 of HVT. Staining of HVT-infected cell culture by Marek's disease herpesvirus (MDHV) antiserum showed intranuclear fluorescence but attempts to prepare HVT precipitating antigen or to demonstrate cross-precipiation between HVT and MDHV were unsuccessful.
从两个鸡群的8只小火鸡中的6只的肾脏中分离出了火鸡疱疹病毒（HVT）。通过合胞体样细胞病理学、5-溴脱氧尿苷对噬斑形成的抑制、细胞培养中A型考德里核内包涵体的形成以及在负染制剂和感染细胞培养物的超薄切片中存在疱疹型病毒粒子来鉴定分离株。将其中一个分离株接种到鸡中，在10周的观察期内证明无致病性。间接免疫荧光和血清中和试验揭示了这些分离株与HVT NSW 1/70株之间的血清学关系。用马立克氏病疱疹病毒（MDHV）抗血清对HVT感染的细胞培养物进行染色显示核内荧光，但制备HVT沉淀抗原或证明HVT与MDHV之间交叉沉淀的尝试均未成功。
