To S C, Moon H W, Runnels P L
Infect Immun. 1984 Jan;43(1):1-5. doi: 10.1128/iai.43.1.1-5.1984.
This study was performed to determine whether the F1 (type 1) pili of a porcine strain of enterotoxigenic Escherichia coli are protective antigens and whether they are produced in the pig small intestine during disease caused by an enterotoxigenic E. coli. Reciprocal cross-absorption experiments with antisera prepared against F1 pili purified from enterotoxigenic E. coli 431 (O101:K30,99:H-:F1) and P14 (O149:K91,88ac:8H+:F1) demonstrated that the F1 antigens of the two strains were closely related or identical. Pregnant swine vaccinated with a vaccine prepared from strain P14 (F1+) responded with a significant increase in antibody against F1 in their serum and colostrum. However, the vaccinated dams did not significantly protect their suckling pigs against fatal challenge with strain 431. There was no evidence of F1 pilus production in the strain 431-infected pigs, as determined by immunofluorescent staining of ileal sections, direct electron microscopic examination of bacteria from ilea, and titration of serum agglutinins in convalescent pigs. It was concluded that strain 431 did not produce F1 in the small intestine during disease and that F1 was not a protective antigen in this system.
进行本研究以确定猪源产肠毒素大肠杆菌的F1（1型）菌毛是否为保护性抗原，以及在产肠毒素大肠杆菌引起的疾病期间它们是否在猪小肠中产生。用针对从产肠毒素大肠杆菌431（O101:K30,99:H-:F1）和P14（O149:K91,88ac:8H+:F1）纯化的F1菌毛制备的抗血清进行交叉吸收实验，结果表明这两种菌株的F1抗原密切相关或相同。用由菌株P14（F1+）制备的疫苗对怀孕母猪进行免疫接种后，其血清和初乳中抗F1抗体显著增加。然而，接种疫苗的母猪并未显著保护其哺乳仔猪免受431菌株的致命攻击。通过回肠切片的免疫荧光染色、回肠细菌的直接电子显微镜检查以及恢复期仔猪血清凝集素滴定，未发现431感染猪中有F1菌毛产生的证据。得出的结论是，431菌株在疾病期间未在小肠中产生F1，且F1在该系统中不是保护性抗原。
