一种用于产前性别鉴定的快速筛查测试。
A rapid screening test for antenatal sex determination.
作者信息
Lau Y F, Huang J C, Dozy A M, Kan Y W
出版信息
Lancet. 1984 Jan 7;1(8367):14-6. doi: 10.1016/s0140-6736(84)90182-x.
PMID:6140342
Abstract
A recombinant DNA probe was used for antenatal sex determination. The probe was isolated from the 3.4 kilobase human repeat sequence derived from heterochromatin of the Y chromosome and had 1000 times more affinity for male DNA than for female DNA. A dot blot hybridisation method with 200 microliter of amniotic fluid yielded accurate results in 2 to 3 days. This test should facilitate antenatal diagnosis of sex-linked genetic disorders.
摘要
一种重组DNA探针被用于产前性别鉴定。该探针是从Y染色体异染色质衍生的3.4千碱基对的人类重复序列中分离出来的，对男性DNA的亲和力比对女性DNA的亲和力高1000倍。采用200微升羊水的斑点杂交法在2至3天内可得出准确结果。这项检测应有助于对伴性遗传病进行产前诊断。
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