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通过PCR扩增SRY和DYZ1 Y染色体特异性序列进行体育赛事中的性别验证：女运动员中存在DYZ1重复序列

Gender verification in sports by PCR amplification of SRY and DYZ1 Y chromosome specific sequences: presence of DYZ1 repeat in female athletes.

作者信息

Serrat A, García de Herreros A

机构信息

Unitat de Biologia Cellular i Molecular, Institut Municipal d'Investigació Mèdica (IMIM), Universitat Autònoma de Barcelona, Spain.

出版信息

Br J Sports Med. 1996 Dec;30(4):310-2. doi: 10.1136/bjsm.30.4.310.

DOI:10.1136/bjsm.30.4.310
PMID:9015592
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1332413/
Abstract

OBJECTIVE

To perform genetic sex typing during the Barcelona Olympic Games using polymerase chain reaction (PCR) amplification of Y chromosome specific sequences.

METHODS

The assay consisted of the amplification of a specific sequence corresponding to the repeat DYZ1 element from buccal smears samples of 2406 female competitors. Positive samples were reanalysed for the presence of another Y chromosome specific gene, SRY.

RESULTS

The expression of these two elements did not always correlate; six samples were found where the presence of DYZ1 but not SRY was detected. This presence of DYZ1 sequence in female athletes is higher than in unselected females, where no DYZ1 amplification was observed in any of the 1629 samples analysed.

CONCLUSIONS

Amplification of DYZ1 repeat should not be used as the only index for determining genetic sex, at least in sporting events.

摘要

目的

在巴塞罗那奥运会期间，采用聚合酶链反应（PCR）扩增Y染色体特异性序列进行遗传性别鉴定。

方法

该检测包括对2406名女性参赛者口腔涂片样本中与重复序列DYZ1元件相对应的特定序列进行扩增。对阳性样本重新分析另一个Y染色体特异性基因SRY的存在情况。

结果

这两个元件的表达并不总是相关；发现6个样本中检测到DYZ1但未检测到SRY。女运动员中DYZ1序列的存在高于未选女性，在所分析的1629个样本中，没有一个观察到DYZ1扩增。

结论

至少在体育赛事中，DYZ1重复序列的扩增不应作为确定遗传性别的唯一指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f335/1332413/385b55c0df42/brjsmed00008-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f335/1332413/a6557fc456b4/brjsmed00008-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f335/1332413/385b55c0df42/brjsmed00008-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f335/1332413/a6557fc456b4/brjsmed00008-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f335/1332413/385b55c0df42/brjsmed00008-0041-a.jpg

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