Kára J, Mach O, Smrt J
Acta Virol. 1983 Nov;27(6):477-83.
Antiviral and antileukaemic effects of the synthetic (2'-5')-oligoadenylate trimer [(2'-5')-ApApA] were demonstrated in BALB/c mice infected with Rauscher murine leukaemia virus (RMLV) by intraperitoneal (i.p.) treatment for 5-20 days (100 micrograms--1 mg daily doses) as evidenced by 72% suppression of viraemia and by decreased activity of serum reverse transcriptase levels. Electron microscopy revealed more than 95% inhibition of RMLV replication as compared to controls in transformed spleen cells from mice treated 5 times with 1 mg dose of (2'-5') ApApA. A significant and dose-dependent reduction of spleen weights of the RMLV-infected mice treated with (2'-5') ApApA was also observed. The antileukaemic effect of (2'-5-') ApApA was enhanced by simultaneous i.p. injection of amphotericin B (20 micrograms/mouse). In comparison to the effect of interferon (IFN) on RNA tumour viruses, our results suggest a higher antiviral activity of the synthetic (2'-5') ApApA oligonucleotide in suppressing RMLV replication in vivo.
合成的（2'-5'）-寡腺苷酸三聚体[（2'-5'）-ApApA]的抗病毒和抗白血病作用在感染劳斯氏小鼠白血病病毒（RMLV）的BALB/c小鼠中得到证实，通过腹腔内（i.p.）治疗5至20天（每日剂量100微克至1毫克），病毒血症抑制72%以及血清逆转录酶水平活性降低可证明这一点。电子显微镜显示，与用1毫克剂量的（2'-5'）ApApA处理5次的小鼠转化脾细胞中的对照相比，RMLV复制的抑制率超过95%。还观察到用（2'-5'）ApApA处理的RMLV感染小鼠的脾脏重量显著且呈剂量依赖性降低。通过同时腹腔注射两性霉素B（20微克/小鼠），（2'-5'）ApApA的抗白血病作用增强。与干扰素（IFN）对RNA肿瘤病毒的作用相比，我们的结果表明合成的（2'-5'）ApApA寡核苷酸在体内抑制RMLV复制方面具有更高的抗病毒活性。
